WNT5A monoclonal antibody, clone 6F2
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis using WNT5A monoclonal antibody, clone 6F2 (Cat # MAB10373) against HEK293 (1) and WNT5A-hIgGFc transfected HEK293 cell lysate (2).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human lung cancer (A), thyroid cancer (B), lymph node (C) and brain (D) showing cytoplasmic and extracellular matrix localization. using WNT5A monoclonal antibody, clone 6F2 (Cat # MAB10373) with DAB staining.
Immunofluorescence
Confocal immunofluorescence analysis of PC-12 cells using WNT5A monoclonal antibody, clone 6F2 (Cat # MAB10373) (green), showing cytoplasmic localization. Blue: DRAQ5 fluorescent DNA dye.
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant WNT5A.
Immunogen
Recombinant protein corresponding to human WNT5A.
Host
Mouse
Theoretical MW (kDa)
42.3
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunofluorescence (1:200-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis using WNT5A monoclonal antibody, clone 6F2 (Cat # MAB10373) against HEK293 (1) and WNT5A-hIgGFc transfected HEK293 cell lysate (2).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human lung cancer (A), thyroid cancer (B), lymph node (C) and brain (D) showing cytoplasmic and extracellular matrix localization. using WNT5A monoclonal antibody, clone 6F2 (Cat # MAB10373) with DAB staining.Immunofluorescence
Confocal immunofluorescence analysis of PC-12 cells using WNT5A monoclonal antibody, clone 6F2 (Cat # MAB10373) (green), showing cytoplasmic localization. Blue: DRAQ5 fluorescent DNA dye.Enzyme-linked Immunoabsorbent Assay
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Gene Info — WNT5A
Entrez GeneID
7474Gene Name
WNT5A
Gene Alias
hWNT5A
Gene Description
wingless-type MMTV integration site family, member 5A
Omim ID
164975Gene Ontology
HyperlinkGene Summary
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 98%, 98% and 87% amino acid identity to the mouse, rat and the xenopus Wnt5A protein, respectively. The experiments performed in Xenopus laevis embryos identified that human frizzled-5 (hFz5) is the receptor for the Wnt5A ligand and the Wnt5A/hFz5 signaling mediates axis induction. [provided by RefSeq
Other Designations
WNT-5A protein
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Interactome
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Pathway
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Disease
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