WNT1 monoclonal antibody, clone 10C8
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis using WNT1 monoclonal antibody, clone 10C8 (Cat # MAB10372) against NIH/3T3 (1), 3T3L1 (2) and HeLa (3) cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human adrenal gland tissue using WNT1 monoclonal antibody, clone 10C8 (Cat # MAB10372) with DAB staining.
Immunofluorescence
Immunofluorescence analysis of HeLa (A) and 3T3-L1 (B) cells using WNT1 monoclonal antibody, clone 10C8 (Cat # MAB10372) (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
Flow Cytometry
Flow cytometric analysis of HeLa cells using WNT1 monoclonal antibody, clone 10C8 (Cat # MAB10372) (green) and negative control (purple).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant WNT1.
Immunogen
Recombinant protein corresponding to human WNT1.
Host
Mouse
Theoretical MW (kDa)
41
Reactivity
Human, Mouse
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunofluorescence (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis using WNT1 monoclonal antibody, clone 10C8 (Cat # MAB10372) against NIH/3T3 (1), 3T3L1 (2) and HeLa (3) cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human adrenal gland tissue using WNT1 monoclonal antibody, clone 10C8 (Cat # MAB10372) with DAB staining.Immunofluorescence
Immunofluorescence analysis of HeLa (A) and 3T3-L1 (B) cells using WNT1 monoclonal antibody, clone 10C8 (Cat # MAB10372) (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of HeLa cells using WNT1 monoclonal antibody, clone 10C8 (Cat # MAB10372) (green) and negative control (purple). -
Gene Info — WNT1
Entrez GeneID
7471Gene Name
WNT1
Gene Alias
INT1
Gene Description
wingless-type MMTV integration site family, member 1
Omim ID
164820Gene Ontology
HyperlinkGene Summary
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It is very conserved in evolution, and the protein encoded by this gene is known to be 98% identical to the mouse Wnt1 protein at the amino acid level. The studies in mouse indicate that the Wnt1 protein functions in the induction of the mesencephalon and cerebellum. This gene was originally considered as a candidate gene for Joubert syndrome, an autosomal recessive disorder with cerebellar hypoplasia as a leading feature. However, further studies suggested that the gene mutations might not have a significant role in Joubert syndrome. This gene is clustered with another family member, WNT10B, in the chromosome 12q13 region. [provided by RefSeq
Other Designations
Wingless-type MMTV integration site family, member 1 (oncogene INT1)
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Interactome
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Pathway
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Disease
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