KLK3 monoclonal antibody, clone 5A11E2
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant KLK3.
Immunogen
Recombinant protein corresponding to amino acids 26-251 of human KLK3.
Host
Mouse
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Recombinant protein)
Western blot analysis using KLK3 monoclonal antibody, clone 5A11E2 (Cat # MAB10358) against recombinant human KLK3 protein.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using KLK3 monoclonal antibody, clone 5A11E2 (Cat # MAB10358) with DAB staining.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human prostate tissue using KLK3 monoclonal antibody, clone 5A11E2 (Cat # MAB10358) with DAB staining.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of PC-3 cells using KLK3 monoclonal antibody, clone 5A11E2 (Cat # MAB10358) (bottom) and negative control (upper). -
Gene Info — KLK3
Entrez GeneID
354Gene Name
KLK3
Gene Alias
APS, KLK2A1, PSA, hK3
Gene Description
kallikrein-related peptidase 3
Omim ID
176820Gene Ontology
HyperlinkGene Summary
Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Its protein product is a protease present in seminal plasma. It is thought to function normally in the liquefaction of seminal coagulum, presumably by hydrolysis of the high molecular mass seminal vesicle protein. Serum level of this protein, called PSA in the clinical setting, is useful in the diagnosis and monitoring of prostatic carcinoma. Alternate splicing of this gene generates several transcript variants encoding different isoforms. [provided by RefSeq
Other Designations
P-30 antigen|gamma-seminoprotein|kallikrein 3, (prostate specific antigen)|prostate specific antigen|semenogelase|seminin
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Interactome
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