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Last updated: 2017/11/19

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PEG10 monoclonal antibody, clone 1B1C4

  • Catalog # : MAB10356
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant PEG10.
  • Immunogen:
  • Recombinant protein corresponding to amino acids 1-120 of human PEG10.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Isotype:
  • IgG1
  • Recommend Usage:
  • ELISA (1:10000)
    Western Blot (1:500-1:2000)
    Immunohistochemistry (1:200-1:1000)
    Immunofluorescence (1:200-1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In ascites (0.03% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot
  • Western Blot
  • Western blot analysis using PEG10 monoclonal antibody, clone 1B1C4 (Cat # MAB10356) against truncated Trx-PEG10 recombinant protein (1), truncated GST-PEG10 (aa 1-120) recombinant protein (2) and full-length PEG10 (aa 1-325)-hIgGFc transfected CHO-K1 cell lysate (3).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical analysis of paraffin-embedded human placenta tissue using PEG10 monoclonal antibody, clone 1B1C4 (Cat # MAB10356) with DAB staining.
  • Immunofluorescence
  • Immunofluorescence
  • Confocal immunofluorescence analysis of HepG2 cells using PEG10 monoclonal antibody, clone 1B1C4 (Cat # MAB10356) (green), showing cytoplasmic localization. Blue: DRAQ5 fluorescent DNA dye.
  • ELISA
  • Application Image
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Gene Name:
  • PEG10
  • Gene Alias:
  • Edr,HB-1,KIAA1051,MEF3L,Mar2,Mart2,RGAG3
  • Gene Description:
  • paternally expressed 10
  • Gene Summary:
  • This gene includes two overlapping reading frames of the same transcript encoding distinct isoforms. The shorter isoform has a CCHC-type zinc finger motif containing a sequence characteristic of gag proteins of most retroviruses and some retrotransposons, and it functions in part by interacting with members of the TGF-beta receptor family. The longer isoform has the active-site DSG consensus sequence of the protease domain of pol proteins. The longer isoform is the result of -1 translational frameshifting that is also seen in some retroviruses. Expression of these two isoforms only comes from the paternal allele due to imprinting. Increased gene expression (as observed by an increase in mRNA levels) is associated with hepatocellular carcinomas. [provided by RefSeq
  • Other Designations:
  • MEF3 like 1,embryonal carcinoma differentiation regulated,retrotransposon gag domain containing 3
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