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PAK2 monoclonal antibody, clone 3B5

  • Catalog # : MAB10355
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against recombinant PAK2.
  • Immunogen:
  • Recombinant protein corresponding to human PAK2.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 61
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Isotype:
  • IgG1
  • Recommend Usage:
  • ELISA (1:10000)
    Western Blot (1:500-1:2000)
    Immunohistochemistry (1:200-1:1000)
    Immunofluorescence (1:200-1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In ascites (0.03% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis using PAK2 monoclonal antibody, clone 3B5 (Cat # MAB10355) against HeLa (1), Jurkat (2), A-549 (3), HEK293 (4) and K-562 (5) cell lysate.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical analysis of paraffin-embedded human lung cancer (A) and gastric cancer (B) using PAK2 monoclonal antibody, clone 3B5 (Cat # MAB10355) with DAB staining.
  • Immunofluorescence
  • Immunofluorescence
  • Confocal immunofluorescence analysis of HeLa cells using PAK2 monoclonal antibody, clone 3B5 (Cat # MAB10355) (green). Blue: DRAQ5 fluorescent DNA dye.
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 5062
  • Gene Name:
  • PAK2
  • Gene Alias:
  • PAK65,PAKgamma
  • Gene Description:
  • p21 protein (Cdc42/Rac)-activated kinase 2
  • Gene Summary:
  • The p21 activated kinases (PAK) are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. The PAK proteins are a family of serine/threonine kinases that serve as targets for the small GTP binding proteins, CDC42 and RAC1, and have been implicated in a wide range of biological activities. The protein encoded by this gene is activated by proteolytic cleavage during caspase-mediated apoptosis, and may play a role in regulating the apoptotic events in the dying cell. [provided by RefSeq
  • Other Designations:
  • S6/H4 kinase,p21 (CDKN1A)-activated kinase 2,p21-activated kinase 2
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