Immunohistochemical analysis of paraffin-embedded human cerebra (A) and lung carcinoma (B) tissue using MDM4 monoclonal antobody, clone 2D10F4 (Cat # MAB10353), showing nuclear localization with DAB staining.
Confocal immunofluorescence analysis of cells using MDM4 monoclonal antobody, clone 2D10F4 (Cat # MAB10353) (green). Red: Actin filaments have been labeled with DY-554 phalloidin.
The human MDM4 gene, which plays a role in apoptosis, encodes a 490-amino acid protein containing a RING finger domain and a putative nuclear localization signal. The MDM4 putative nuclear localization signal, which all Mdm proteins contain, is located in the C-terminal region of the protein. The mRNA is expressed at a high level in thymus and at lower levels in all other tissues tested. MDM4 protein produced by in vitro translation interacts with p53 via a binding domain located in the N-terminal region of the MDM4 protein. MDM4 shows significant structural similarity to p53-binding protein MDM2. Two transcript variants, one protein-coding and the other likely not to be protein-coding, have been found for this gene. [provided by RefSeq
MDM4-related protein 1,Mdm4, transformed 3T3 cell double minute 4, p53 binding protein,double minute 4, human homolog of; p53-binding protein,mouse double minute 4 homolog,p53-binding protein