GPI monoclonal antibody, clone 1B7D7
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis using GPI monoclonal antobody, clone 1B7D7 (Cat # MAB10351) against HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY GPI cDNA (2).
Western Blot
Western blot analysis using GPI monoclonal antobody, clone 1B7D7 (Cat # MAB10351) against HepG2 (1) , SMMC-7721 (2) cell lysate and rat liver tissue lysate (3).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human kidney tissue using GPI monoclonal antobody, clone 1B7D7 (Cat # MAB10351) with DAB staining.
Immunofluorescence
Confocal immunofluorescence analysis of L-02 cells using GPI monoclonal antobody, clone 1B7D7 (Cat # MAB10351) (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant GPI.
Immunogen
Recombinant protein corresponding to human GPI.
Host
Mouse
Theoretical MW (kDa)
63
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunofluorescence (1:200-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis using GPI monoclonal antobody, clone 1B7D7 (Cat # MAB10351) against HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY GPI cDNA (2).Western Blot
Western blot analysis using GPI monoclonal antobody, clone 1B7D7 (Cat # MAB10351) against HepG2 (1) , SMMC-7721 (2) cell lysate and rat liver tissue lysate (3).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human kidney tissue using GPI monoclonal antobody, clone 1B7D7 (Cat # MAB10351) with DAB staining.Immunofluorescence
Confocal immunofluorescence analysis of L-02 cells using GPI monoclonal antobody, clone 1B7D7 (Cat # MAB10351) (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.Enzyme-linked Immunoabsorbent Assay
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Gene Info — GPI
Entrez GeneID
2821Gene Name
GPI
Gene Alias
AMF, GNPI, NLK, PGI, PHI, SA-36
Gene Description
glucose phosphate isomerase
Omim ID
172400Gene Ontology
HyperlinkGene Summary
This gene belongs to the GPI family whose members encode multifunctional phosphoglucose isomerase proteins involved in energy pathways. The protein encoded by this gene is a dimeric enzyme that catalyzes the reversible isomerization of glucose-6-phosphate and fructose-6-phosphate. The protein functions in different capacities inside and outside the cell. In the cytoplasm, the gene product is involved in glycolysis and gluconeogenesis, while outside the cell it functions as a neurotrophic factor for spinal and sensory neurons. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment. [provided by RefSeq
Other Designations
autocrine motility factor|glucose-6-phosphate isomerase|hexose monophosphate isomerase|hexosephosphate isomerase|neuroleukin|oxoisomerase|phosphoglucose isomerase|phosphohexomutase|phosphohexose isomerase|phosphosaccharomutase|sperm antigen-36
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Interactome
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Pathway
- Amino sugar and nucleotide sugar metabolism
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from ornithine
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Glycolysis / Gluconeogenesis
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