WNT10B monoclonal antibody, clone 5A7
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant WNT10B.
Immunogen
Recombinant protein corresponding to human WNT10B.
Host
Mouse
Theoretical MW (kDa)
43
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunofluorescence (1:200-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis using WNT10B monoclonal antobody, clone 5A7 (Cat # MAB10347) against HeLa cell extracts.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human normal stomach (A), normal liver (B), normal kidney (C) and rectum cancer tissue (D) using WNT10B monoclonal antobody, clone 5A7 (Cat # MAB10347) with DAB staining.Immunofluorescence
Confocal immunofluorescence analysis of PANC-1 cells using WNT10B monoclonal antobody, clone 5A7 (Cat # MAB10347) (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Blue: DRAQ5 fluorescent DNA dye.Enzyme-linked Immunoabsorbent Assay
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Gene Info — WNT10B
Entrez GeneID
7480Gene Name
WNT10B
Gene Alias
SHFM6, WNT-12
Gene Description
wingless-type MMTV integration site family, member 10B
Omim ID
601906Gene Ontology
HyperlinkGene Summary
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It may be involved in breast cancer, and its protein signaling is likely a molecular switch that governs adipogenesis. This protein is 96% identical to the mouse Wnt10b protein at the amino acid level. This gene is clustered with another family member, WNT1, in the chromosome 12q13 region. [provided by RefSeq
Other Designations
WNT-10B protein
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Interactome
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