WIF1 monoclonal antibody, clone 1G5
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis using WIF1 monoclonal antobody, clone 1G5 (Cat # MAB10346) against HeLa (1), NIH/3T3 (2) and NTERA-2 (3) cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human ovary tumour tissue (A) and lung cancer (B) using WIF1 monoclonal antobody, clone 1G5 (Cat # MAB10346) with DAB staining.
Immunofluorescence
Immunofluorescence analysis of HeLa cells using WIF1 monoclonal antobody, clone 1G5 (Cat # MAB10346) (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant WIF1.
Immunogen
Recombinant protein corresponding to human WIF1.
Host
Mouse
Theoretical MW (kDa)
42
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunofluorescence (1:200-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis using WIF1 monoclonal antobody, clone 1G5 (Cat # MAB10346) against HeLa (1), NIH/3T3 (2) and NTERA-2 (3) cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human ovary tumour tissue (A) and lung cancer (B) using WIF1 monoclonal antobody, clone 1G5 (Cat # MAB10346) with DAB staining.Immunofluorescence
Immunofluorescence analysis of HeLa cells using WIF1 monoclonal antobody, clone 1G5 (Cat # MAB10346) (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Enzyme-linked Immunoabsorbent Assay
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Gene Info — WIF1
Entrez GeneID
11197Gene Name
WIF1
Gene Alias
WIF-1
Gene Description
WNT inhibitory factor 1
Omim ID
605186Gene Ontology
HyperlinkGene Summary
WNT proteins are extracellular signaling molecules involved in the control of embryonic development. This gene encodes a secreted protein, which binds WNT proteins and inhibits their activities. This protein contains a WNT inhibitory factor (WIF) domain and 5 epidermal growth factor (EGF)-like domains. It may be involved in mesoderm segmentation. This protein is found to be present in fish, amphibia and mammals. [provided by RefSeq
Other Designations
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Interactome
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Pathway
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Disease
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