TUBB3 monoclonal antibody, clone 2E9
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis using TUBB3 monoclonal antobody, clone 2E9 (Cat # MAB10341) against HepG2 (1), A-549 (2) and HeLa (3) cell lysate.
Immunofluorescence
Immunofluorescence analysis of PANC-1 cells using TUBB3 monoclonal antobody, clone 2E9 (Cat # MAB10341) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow Cytometry
Flow cytometric analysis of A-549 cells using TUBB3 monoclonal antobody, clone 2E9 (Cat # MAB10341) (green) and negative control (purple).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant TUBB3.
Immunogen
Recombinant protein corresponding to human TUBB3.
Host
Mouse
Theoretical MW (kDa)
50
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunofluorescence (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis using TUBB3 monoclonal antobody, clone 2E9 (Cat # MAB10341) against HepG2 (1), A-549 (2) and HeLa (3) cell lysate.Immunofluorescence
Immunofluorescence analysis of PANC-1 cells using TUBB3 monoclonal antobody, clone 2E9 (Cat # MAB10341) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of A-549 cells using TUBB3 monoclonal antobody, clone 2E9 (Cat # MAB10341) (green) and negative control (purple). -
Gene Info — TUBB3
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Interactome
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Pathway
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