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Last updated: 2016/12/4
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SKP1 monoclonal antibody, clone 1H9

  • Catalog # : MAB10320
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against recombinant SKP1.
  • Immunogen:
  • Recombinant protein corresponding to human SKP1.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 19
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Recommend Usage:
  • ELISA (1:10000)
    Western Blot (1:500-1:2000)
    Immunohistochemistry (1:200-1:1000)
    Flow cytometry (1:200-1:400)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In ascites (0.03% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis using SKP1 monoclonal antobody, clone 1H9 (Cat # MAB10320) against HeLa (1), RAJI (2), Jurkat (3), MCF-7 (4), HepG2 (5), PC-12 (6) and COS-7 (7) cell lysate.
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • Western blot analysis using SKP1 monoclonal antobody, clone 1H9 (Cat # MAB10320) against recombinant human SKP1 protein.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue (A) and cervical cancer tissue (B) using SKP1 monoclonal antobody, clone 1H9 (Cat # MAB10320) with DAB staining.
  • ELISA
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of HeLa cells using SKP1 monoclonal antobody, clone 1H9 (Cat # MAB10320) (green) and negative control (red).
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 6500
  • Gene Name:
  • SKP1
  • Gene Alias:
  • EMC19,MGC34403,OCP-II,OCP2,SKP1A,TCEB1L,p19A
  • Gene Description:
  • S-phase kinase-associated protein 1
  • Gene Summary:
  • This gene encodes a component of SCF complexes, which are composed of this protein, cullin 1, a ring-box protein, and one member of the F-box family of proteins. This protein binds directly to the F-box motif found in F-box proteins. SCF complexes are involved in the regulated ubiquitination of specific protein substrates, which targets them for degradation by the proteosome. Specific F-box proteins recognize different target protein(s), and many specific SCF substrates have been identified including regulators of cell cycle progression and development. Studies have also characterized the protein as an RNA polymerase II elongation factor. Alternative splicing of this gene results in two transcript variants. A related pseudogene has been identified on chromosome 7. [provided by RefSeq
  • Other Designations:
  • RNA polymerase II elongation factor-like protein OCP2,cyclin A/CDK2-associated p19,organ of Corti protein 2,transcription elongation factor B (SIII), polypeptide 1-like
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