SKP1 monoclonal antibody, clone 4E11
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis using SKP1 monoclonal antobody, clone 4E11 (Cat # MAB10319) against HeLa (1), NIH/3T3 (2), A-431 (3), RAJI (4), PC-12 (5), COS-7 (6), MCF-7 (7) and HepG2 (8) cell lysate.
Western Blot (Recombinant protein)
Western blot analysis using SKP1 monoclonal antobody, clone 4E11 (Cat # MAB10319) against recombinant human SKP1 protein.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue using SKP1 monoclonal antobody, clone 4E11 (Cat # MAB10319) with DAB staining.
Immunofluorescence
Immunofluorescence analysis of HeLa cells using SKP1 monoclonal antobody, clone 4E11 (Cat # MAB10319) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow Cytometry
Flow cytometric analysis of NIH/3T3 cells using SKP1 monoclonal antobody, clone 4E11 (Cat # MAB10319) (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant SKP1.
Immunogen
Recombinant protein corresponding to human SKP1.
Host
Mouse
Theoretical MW (kDa)
19
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunofluorescence (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis using SKP1 monoclonal antobody, clone 4E11 (Cat # MAB10319) against HeLa (1), NIH/3T3 (2), A-431 (3), RAJI (4), PC-12 (5), COS-7 (6), MCF-7 (7) and HepG2 (8) cell lysate.Western Blot (Recombinant protein)
Western blot analysis using SKP1 monoclonal antobody, clone 4E11 (Cat # MAB10319) against recombinant human SKP1 protein.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue using SKP1 monoclonal antobody, clone 4E11 (Cat # MAB10319) with DAB staining.Immunofluorescence
Immunofluorescence analysis of HeLa cells using SKP1 monoclonal antobody, clone 4E11 (Cat # MAB10319) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of NIH/3T3 cells using SKP1 monoclonal antobody, clone 4E11 (Cat # MAB10319) (green) and negative control (red). -
Gene Info — SKP1
Entrez GeneID
6500Gene Name
SKP1
Gene Alias
EMC19, MGC34403, OCP-II, OCP2, SKP1A, TCEB1L, p19A
Gene Description
S-phase kinase-associated protein 1
Omim ID
601434Gene Ontology
HyperlinkGene Summary
This gene encodes a component of SCF complexes, which are composed of this protein, cullin 1, a ring-box protein, and one member of the F-box family of proteins. This protein binds directly to the F-box motif found in F-box proteins. SCF complexes are involved in the regulated ubiquitination of specific protein substrates, which targets them for degradation by the proteosome. Specific F-box proteins recognize different target protein(s), and many specific SCF substrates have been identified including regulators of cell cycle progression and development. Studies have also characterized the protein as an RNA polymerase II elongation factor. Alternative splicing of this gene results in two transcript variants. A related pseudogene has been identified on chromosome 7. [provided by RefSeq
Other Designations
RNA polymerase II elongation factor-like protein OCP2|cyclin A/CDK2-associated p19|organ of Corti protein 2|transcription elongation factor B (SIII), polypeptide 1-like
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Interactome
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Pathway
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Disease
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