Mouse monoclonal antibody raised against recombinant SKP1.
Recombinant protein corresponding to human SKP1.
Theoretical MW (kDa):
ELISA (1:10000) Western Blot (1:500-1:2000) Immunohistochemistry (1:200-1:1000) Immunofluorescence (1:200-1:1000) Flow cytometry (1:200-1:400) The optimal working dilution should be determined by the end user.
In ascites (0.03% sodium azide)
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue using SKP1 monoclonal antobody, clone 4E11 (Cat # MAB10319) with DAB staining.
Immunofluorescence analysis of HeLa cells using SKP1 monoclonal antobody, clone 4E11 (Cat # MAB10319) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow cytometric analysis of NIH/3T3 cells using SKP1 monoclonal antobody, clone 4E11 (Cat # MAB10319) (green) and negative control (red).
This gene encodes a component of SCF complexes, which are composed of this protein, cullin 1, a ring-box protein, and one member of the F-box family of proteins. This protein binds directly to the F-box motif found in F-box proteins. SCF complexes are involved in the regulated ubiquitination of specific protein substrates, which targets them for degradation by the proteosome. Specific F-box proteins recognize different target protein(s), and many specific SCF substrates have been identified including regulators of cell cycle progression and development. Studies have also characterized the protein as an RNA polymerase II elongation factor. Alternative splicing of this gene results in two transcript variants. A related pseudogene has been identified on chromosome 7. [provided by RefSeq
RNA polymerase II elongation factor-like protein OCP2,cyclin A/CDK2-associated p19,organ of Corti protein 2,transcription elongation factor B (SIII), polypeptide 1-like