SIRT1 monoclonal antibody, clone 1F3
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis using SIRT1 monoclonal antobody, clone 1F3 (Cat # MAB10318) against MCF-7 (1), Jurkat (2), HeLa (3), HEK293 (4) and A-549 (5) cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue (A) and kidney cancer tissue (B) using SIRT1 monoclonal antobody, clone 1F3 (Cat # MAB10318) with DAB staining.
Immunofluorescence
Immunofluorescence analysis of NTERA-2 cells using SIRT1 monoclonal antobody, clone 1F3 (Cat # MAB10318) (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow Cytometry
Flow cytometric analysis of K-562 cells using SIRT1 monoclonal antobody, clone 1F3 (Cat # MAB10318) (green) and negative control (purple).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant SIRT1.
Immunogen
Recombinant protein corresponding to human SIRT1.
Host
Mouse
Theoretical MW (kDa)
120
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunofluorescence (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis using SIRT1 monoclonal antobody, clone 1F3 (Cat # MAB10318) against MCF-7 (1), Jurkat (2), HeLa (3), HEK293 (4) and A-549 (5) cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue (A) and kidney cancer tissue (B) using SIRT1 monoclonal antobody, clone 1F3 (Cat # MAB10318) with DAB staining.Immunofluorescence
Immunofluorescence analysis of NTERA-2 cells using SIRT1 monoclonal antobody, clone 1F3 (Cat # MAB10318) (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of K-562 cells using SIRT1 monoclonal antobody, clone 1F3 (Cat # MAB10318) (green) and negative control (purple). -
Gene Info — SIRT1
Entrez GeneID
23411Gene Name
SIRT1
Gene Alias
SIR2L1
Gene Description
sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae)
Omim ID
604479Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants. [provided by RefSeq
Other Designations
OTTHUMP00000019691|OTTHUMP00000060745|SIR2alpha|sir2-like 1|sirtuin 1|sirtuin type 1
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Interactome
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Disease
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