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Last updated: 2017/4/23
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RPS6KA3 monoclonal antibody, clone 4E10

  • Catalog # : MAB10316
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against recombinant RPS6KA3.
  • Immunogen:
  • Recombinant protein corresponding to human RPS6KA3.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 80
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Isotype:
  • IgG1
  • Recommend Usage:
  • ELISA (1:10000)
    Western Blot (1:500-1:2000)
    Immunofluorescence (1:200-1:1000)
    Flow cytometry (1:200-1:400)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In ascites (0.03% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis using RPS6KA3 monoclonal antobody, clone 4E10 (Cat # MAB10316) against HeLa (1), MCF-7 (2), and HepG2 (3) cell lysate.
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • Western blot analysis using RPS6KA3 monoclonal antobody, clone 4E10 (Cat # MAB10316) against recombinant human RPS6KA3 protein.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence analysis of HepG2 cells using RPS6KA3 monoclonal antobody, clone 4E10 (Cat # MAB10316) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • ELISA
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of HepG2 cells using RPS6KA3 monoclonal antobody, clone 4E10 (Cat # MAB10316) (green) and negative control (red).
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 6197
  • Gene Name:
  • RPS6KA3
  • Gene Alias:
  • CLS,HU-3,ISPK-1,MAPKAPK1B,MRX19,RSK,RSK2,S6K-alpha3,p90-RSK2,pp90RSK2
  • Gene Description:
  • ribosomal protein S6 kinase, 90kDa, polypeptide 3
  • Gene Summary:
  • This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Mutations in this gene have been associated with Coffin-Lowry syndrome (CLS). [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000023036,insulin-stimulated protein kinase 1,mental retardation, X-linked 19,ribosomal protein S6 kinase, 90kD, polypeptide 3
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