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Last updated: 2017/12/17
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RICTOR monoclonal antibody, clone 7B3

  • Catalog # : MAB10313
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against recombinant RICTOR.
  • Immunogen:
  • Recombinant protein corresponding to human RICTOR.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 192
  • Reactivity:
  • Human, Mouse
  • Form:
  • Liquid
  • Isotype:
  • IgG1
  • Recommend Usage:
  • ELISA (1:10000)
    Western Blot (1:500-1:2000)
    Immunohistochemistry (1:200-1:1000)
    Immunofluorescence (1:200-1:1000)
    Flow cytometry (1:200-1:400)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In ascites (0.03% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis using RICTOR monoclonal antobody, clone 7B3 (Cat # MAB10313) against HeLa (1), PANC-1 (2), MOLT4 (3), HepG2 (4) and HEK293 (5) cell lysate.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue (A) and breast carcinoma (B) using RICTOR monoclonal antobody, clone 7B3 (Cat # MAB10313) with DAB staining.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence analysis of NIH/3T3 cells using RICTOR monoclonal antobody, clone 7B3 (Cat # MAB10313) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • ELISA
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of HeLa cells using RICTOR monoclonal antobody, clone 7B3 (Cat # MAB10313) (green) and negative control (purple).
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Gene Name:
  • RICTOR
  • Gene Alias:
  • DKFZp686B11164,KIAA1999,MGC39830,mAVO3
  • Gene Description:
  • rapamycin-insensitive companion of mTOR
  • Gene Summary:
  • RICTOR and MTOR (FRAP1; MIM 601231) are components of a protein complex that integrates nutrient- and growth factor-derived signals to regulate cell growth (Sarbassov et al., 2004 [PubMed 15268862]).[supplied by OMIM
  • Other Designations:
  • TORC2-specific protein AVO3,pianissimo
  • Gene Pathway
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