Mouse monoclonal antibody raised against recombinant RICTOR.
Recombinant protein corresponding to human RICTOR.
Theoretical MW (kDa):
ELISA (1:10000) Western Blot (1:500-1:2000) Immunohistochemistry (1:200-1:1000) Immunofluorescence (1:200-1:1000) Flow cytometry (1:200-1:400) The optimal working dilution should be determined by the end user.
In ascites (0.03% sodium azide)
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue (A) and breast carcinoma (B) using RICTOR monoclonal antobody, clone 7B3 (Cat # MAB10313) with DAB staining.
Immunofluorescence analysis of NIH/3T3 cells using RICTOR monoclonal antobody, clone 7B3 (Cat # MAB10313) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow cytometric analysis of HeLa cells using RICTOR monoclonal antobody, clone 7B3 (Cat # MAB10313) (green) and negative control (purple).
RICTOR and MTOR (FRAP1; MIM 601231) are components of a protein complex that integrates nutrient- and growth factor-derived signals to regulate cell growth (Sarbassov et al., 2004 [PubMed 15268862]).[supplied by OMIM