RICTOR monoclonal antibody, clone 7B3
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis using RICTOR monoclonal antobody, clone 7B3 (Cat # MAB10313) against HeLa (1), PANC-1 (2), MOLT4 (3), HepG2 (4) and HEK293 (5) cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue (A) and breast carcinoma (B) using RICTOR monoclonal antobody, clone 7B3 (Cat # MAB10313) with DAB staining.
Immunofluorescence
Immunofluorescence analysis of NIH/3T3 cells using RICTOR monoclonal antobody, clone 7B3 (Cat # MAB10313) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow Cytometry
Flow cytometric analysis of HeLa cells using RICTOR monoclonal antobody, clone 7B3 (Cat # MAB10313) (green) and negative control (purple).
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Specifications
Product Description
Mouse monoclonal antibody raised against recombinant RICTOR.
Immunogen
Recombinant protein corresponding to human RICTOR.
Host
Mouse
Theoretical MW (kDa)
192
Reactivity
Human, Mouse
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunofluorescence (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis using RICTOR monoclonal antobody, clone 7B3 (Cat # MAB10313) against HeLa (1), PANC-1 (2), MOLT4 (3), HepG2 (4) and HEK293 (5) cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue (A) and breast carcinoma (B) using RICTOR monoclonal antobody, clone 7B3 (Cat # MAB10313) with DAB staining.Immunofluorescence
Immunofluorescence analysis of NIH/3T3 cells using RICTOR monoclonal antobody, clone 7B3 (Cat # MAB10313) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of HeLa cells using RICTOR monoclonal antobody, clone 7B3 (Cat # MAB10313) (green) and negative control (purple). -
Gene Info — RICTOR
Entrez GeneID
253260Gene Name
RICTOR
Gene Alias
DKFZp686B11164, KIAA1999, MGC39830, mAVO3
Gene Description
rapamycin-insensitive companion of mTOR
Omim ID
609022Gene Ontology
HyperlinkGene Summary
RICTOR and MTOR (FRAP1; MIM 601231) are components of a protein complex that integrates nutrient- and growth factor-derived signals to regulate cell growth (Sarbassov et al., 2004 [PubMed 15268862]).[supplied by OMIM
Other Designations
TORC2-specific protein AVO3|pianissimo
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Interactomes
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Pathways
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Diseases
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