RICTOR monoclonal antibody, clone 7B3

Catalog # MAB10313

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Size:100 uL
Price: USD $ 531.00
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Contact Info
  • +1-909-264-1399
    +1-909-992-0619
    Toll Free : +1-877-853-6098
  • +1-909-992-3401
Images
Western Blot (Cell lysate)
Application

Western Blot (Cell lysate)

Western blot analysis using RICTOR monoclonal antobody, clone 7B3 (Cat # MAB10313) against HeLa (1), PANC-1 (2), MOLT4 (3), HepG2 (4) and HEK293 (5) cell lysate.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue (A) and breast carcinoma (B) using RICTOR monoclonal antobody, clone 7B3 (Cat # MAB10313) with DAB staining.

Immunofluorescence
Application

Immunofluorescence

Immunofluorescence analysis of NIH/3T3 cells using RICTOR monoclonal antobody, clone 7B3 (Cat # MAB10313) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Flow Cytometry
Application

Flow Cytometry

Flow cytometric analysis of HeLa cells using RICTOR monoclonal antobody, clone 7B3 (Cat # MAB10313) (green) and negative control (purple).

  • Specifications

    Product Description

    Mouse monoclonal antibody raised against recombinant RICTOR.

    Immunogen

    Recombinant protein corresponding to human RICTOR.

    Host

    Mouse

    Theoretical MW (kDa)

    192

    Reactivity

    Human, Mouse

    Form

    Liquid

    Isotype

    IgG1

    Recommend Usage

    ELISA (1:10000)
    Western Blot (1:500-1:2000)
    Immunohistochemistry (1:200-1:1000)
    Immunofluorescence (1:200-1:1000)
    Flow cytometry (1:200-1:400)
    The optimal working dilution should be determined by the end user.

    Storage Buffer

    In ascites (0.03% sodium azide)

    Storage Instruction

    Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.

    Note

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

  • Applications

    Western Blot (Cell lysate)

    Western blot analysis using RICTOR monoclonal antobody, clone 7B3 (Cat # MAB10313) against HeLa (1), PANC-1 (2), MOLT4 (3), HepG2 (4) and HEK293 (5) cell lysate.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

    Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue (A) and breast carcinoma (B) using RICTOR monoclonal antobody, clone 7B3 (Cat # MAB10313) with DAB staining.

    Immunofluorescence

    Immunofluorescence analysis of NIH/3T3 cells using RICTOR monoclonal antobody, clone 7B3 (Cat # MAB10313) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

    Enzyme-linked Immunoabsorbent Assay

    Flow Cytometry

    Flow cytometric analysis of HeLa cells using RICTOR monoclonal antobody, clone 7B3 (Cat # MAB10313) (green) and negative control (purple).
  • Gene Info — RICTOR

    Entrez GeneID

    253260

    Gene Name

    RICTOR

    Gene Alias

    DKFZp686B11164, KIAA1999, MGC39830, mAVO3

    Gene Description

    rapamycin-insensitive companion of mTOR

    Omim ID

    609022

    Gene Ontology

    Hyperlink

    Gene Summary

    RICTOR and MTOR (FRAP1; MIM 601231) are components of a protein complex that integrates nutrient- and growth factor-derived signals to regulate cell growth (Sarbassov et al., 2004 [PubMed 15268862]).[supplied by OMIM

    Other Designations

    TORC2-specific protein AVO3|pianissimo

  • Interactomes
  • Pathways
  • Diseases
Contact Info
  • +1-909-264-1399
    +1-909-992-0619
    Toll Free : +1-877-853-6098
  • +1-909-992-3401
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