PTH1R monoclonal antibody, clone 4D2
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis using PTH1R monoclonal antobody, clone 4D2 (Cat # MAB10307) against PTH1R-hIgGFc transfected HEK293 cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human kidney tissue using PTH1R monoclonal antobody, clone 4D2 (Cat # MAB10307) with DAB staining.
Immunofluorescence
Immunofluorescence analysis of SK-BR-3 cells using PTH1R monoclonal antobody, clone 4D2 (Cat # MAB10307) (green). Blue: DRAQ5 fluorescent DNA dye.
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant PTH1R.
Immunogen
Recombinant protein corresponding to human PTH1R.
Host
Mouse
Theoretical MW (kDa)
66
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunofluorescence (1:200-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis using PTH1R monoclonal antobody, clone 4D2 (Cat # MAB10307) against PTH1R-hIgGFc transfected HEK293 cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human kidney tissue using PTH1R monoclonal antobody, clone 4D2 (Cat # MAB10307) with DAB staining.Immunofluorescence
Immunofluorescence analysis of SK-BR-3 cells using PTH1R monoclonal antobody, clone 4D2 (Cat # MAB10307) (green). Blue: DRAQ5 fluorescent DNA dye.Enzyme-linked Immunoabsorbent Assay
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Gene Info — PTH1R
Entrez GeneID
5745Gene Name
PTH1R
Gene Alias
MGC138426, MGC138452, PTHR, PTHR1
Gene Description
parathyroid hormone 1 receptor
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the G-protein coupled receptor family 2. This protein is a receptor for parathyroid hormone (PTH) and for parathyroid hormone-like hormone (PTHLH). The activity of this receptor is mediated by G proteins which activate adenylyl cyclase and also a phosphatidylinositol-calcium second messenger system. Defects in this receptor are known to be the cause of Jansen's metaphyseal chondrodysplasia (JMC), chondrodysplasia Blomstrand type (BOCD), as well as enchodromatosis. [provided by RefSeq
Other Designations
PTH/PTHr receptor|PTH/PTHrP type I receptor|parathyroid hormone receptor 1|parathyroid hormone/parathyroid hormone-related peptide receptor|parathyroid hormone/parathyroid hormone-related protein receptor|seven transmembrane helix receptor
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Interactome
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Pathway
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Disease
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