PKN2 monoclonal antibody, clone 1D1
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant PKN2.
Immunogen
Recombinant protein corresponding to human PKN2.
Host
Mouse
Theoretical MW (kDa)
140
Reactivity
Human, Mouse, Rat
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis using PKN2 monoclonal antobody, clone 1D1 (Cat # MAB10301) against PC-12 (1), COS-7 (2), K-562 (3), Jurkat (4), HeLa (5), A-431 (6), C6 (7), NIH/3T3 (8) and HEK293 (9) cell lysate.Western Blot (Recombinant protein)
Western blot analysis using PKN2 monoclonal antobody, clone 1D1 (Cat # MAB10301) against recombinant human PKN2 protein.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human prostate tissue (A) and lung cancer tissue (B) using PKN2 monoclonal antobody, clone 1D1 (Cat # MAB10301) with DAB staining.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of NIH/3T3 cells using PKN2 monoclonal antobody, clone 1D1 (Cat # MAB10301) (blue) and negative control (red). -
Gene Info — PKN2
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Interactome
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Disease
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