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Last updated: 2017/1/22
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PPP2R4 monoclonal antibody, clone 4D9

  • Catalog # : MAB10300
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against recombinant PPP2R4.
  • Immunogen:
  • Recombinant protein corresponding to human PPP2R4.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 41
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Isotype:
  • IgG1
  • Recommend Usage:
  • ELISA (1:10000)
    Western Blot (1:500-1:2000)
    Immunohistochemistry (1:200-1:1000)
    Immunofluorescence (1:200-1:1000)
    Flow cytometry (1:200-1:400)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.05% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • Western blot analysis using PPP2R4 monoclonal antobody, clone 4D9 (Cat # MAB10300) against recombinant human PPP2R4 protein.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue (A) and lung cancer tissue (B) using PPP2R4 monoclonal antobody, clone 4D9 (Cat # MAB10300) with DAB staining.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence analysis of HeLa cells using PPP2R4 monoclonal antobody, clone 4D9 (Cat # MAB10300) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • ELISA
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of MCF-7 cells using PPP2R4 monoclonal antobody, clone 4D9 (Cat # MAB10300) (green) and negative control (red).
  • Application Image
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 5524
  • Gene Name:
  • PPP2R4
  • Gene Alias:
  • MGC2184,PP2A,PR53,PTPA
  • Gene Description:
  • protein phosphatase 2A activator, regulatory subunit 4
  • Gene Summary:
  • Protein phosphatase 2A is one of the four major Ser/Thr phosphatases and is implicated in the negative control of cell growth and division. Protein phosphatase 2A holoenzymes are heterotrimeric proteins composed of a structural subunit A, a catalytic subunit C, and a regulatory subunit B. The regulatory subunit is encoded by a diverse set of genes that have been grouped into the B/PR55, B'/PR61, and B''/PR72 families. These different regulatory subunits confer distinct enzymatic specificities and intracellular localizations to the holozenzyme. The product of this gene belongs to the B' family. This gene encodes a specific phosphotyrosyl phosphatase activator of the dimeric form of protein phosphatase 2A. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000022333,PP2A phosphatase activator,PP2A, subunit B',phosphotyrosyl phosphatase activator,protein phosphatase 2A, regulatory subunit B',protein phosphatase 2A, regulatory subunit B' (PR 53)
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