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Last updated: 2017/2/19
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PODXL monoclonal antibody, clone 5F5

  • Catalog # : MAB10299
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against recombinant PODXL.
  • Immunogen:
  • Recombinant protein corresponding to human PODXL.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 49
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Isotype:
  • IgG1
  • Recommend Usage:
  • ELISA (1:10000)
    Western Blot (1:500-1:2000)
    Immunohistochemistry (1:200-1:1000)
    Flow cytometry (1:200-1:400)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In ascites (0.03% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • Western blot analysis using PODXL monoclonal antobody, clone 5F5 (Cat # MAB10299) against recombinant human PODXL protein.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical analysis of paraffin-embedded human lung cancer tissue (A) and kidney tissue (B) using PODXL monoclonal antobody, clone 5F5 (Cat # MAB10299) with DAB staining.
  • ELISA
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of HeLa cells using PODXL monoclonal antobody, clone 5F5 (Cat # MAB10299) (green) and negative control (purple).
  • Application Image
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 5420
  • Gene Name:
  • PODXL
  • Gene Alias:
  • Gp200,MGC138240,PC,PCLP
  • Gene Description:
  • podocalyxin-like
  • Gene Summary:
  • This gene encodes a member of the sialomucin protein family. The encoded protein was originally identified as an important component of glomerular podocytes. Podocytes are highly differentiated epithelial cells with interdigitating foot processes covering the outer aspect of the glomerular basement membrane. Other biological activities of the encoded protein include: binding in a membrane protein complex with Na+/H+ exchanger regulatory factor to intracellular cytoskeletal elements, playing a role in hematopoetic cell differentiation, and being expressed in vascular endothelium cells and binding to L-selectin. [provided by RefSeq
  • Other Designations:
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