PODXL monoclonal antibody, clone 5F5
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More Files
- More Functions
Western Blot (Recombinant protein)
Western blot analysis using PODXL monoclonal antobody, clone 5F5 (Cat # MAB10299) against recombinant human PODXL protein.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue (A) and kidney tissue (B) using PODXL monoclonal antobody, clone 5F5 (Cat # MAB10299) with DAB staining.
Flow Cytometry
Flow cytometric analysis of HeLa cells using PODXL monoclonal antobody, clone 5F5 (Cat # MAB10299) (green) and negative control (purple).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant PODXL.
Immunogen
Recombinant protein corresponding to human PODXL.
Host
Mouse
Theoretical MW (kDa)
49
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Recombinant protein)
Western blot analysis using PODXL monoclonal antobody, clone 5F5 (Cat # MAB10299) against recombinant human PODXL protein.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue (A) and kidney tissue (B) using PODXL monoclonal antobody, clone 5F5 (Cat # MAB10299) with DAB staining.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of HeLa cells using PODXL monoclonal antobody, clone 5F5 (Cat # MAB10299) (green) and negative control (purple). -
Gene Info — PODXL
Entrez GeneID
5420Gene Name
PODXL
Gene Alias
Gp200, MGC138240, PC, PCLP
Gene Description
podocalyxin-like
Omim ID
602632Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the sialomucin protein family. The encoded protein was originally identified as an important component of glomerular podocytes. Podocytes are highly differentiated epithelial cells with interdigitating foot processes covering the outer aspect of the glomerular basement membrane. Other biological activities of the encoded protein include: binding in a membrane protein complex with Na+/H+ exchanger regulatory factor to intracellular cytoskeletal elements, playing a role in hematopoetic cell differentiation, and being expressed in vascular endothelium cells and binding to L-selectin. [provided by RefSeq
Other Designations
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Interactome
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Disease
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