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Last updated: 2017/7/23
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LIMS1 monoclonal antibody, clone 5G7

  • Catalog # : MAB10297
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against recombinant LIMS1.
  • Immunogen:
  • Recombinant protein corresponding to human LIMS1.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 37
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Isotype:
  • IgG1
  • Recommend Usage:
  • ELISA (1:10000)
    Western Blot (1:500-1:2000)
    Immunofluorescence (1:200-1:1000)
    Flow cytometry (1:200-1:400)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In ascites (0.03% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis using LIMS1 monoclonal antobody, clone 5G7 (Cat # MAB10297) against A-549 (1), Jurkat (2), and HeLa (3) cell lysate.
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • Western blot analysis using LIMS1 monoclonal antobody, clone 5G7 (Cat # MAB10297) against recombinant human LIMS1 protein.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence analysis of HepG2 cells using LIMS1 monoclonal antobody, clone 5G7 (Cat # MAB10297) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • ELISA
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of HeLa cells using LIMS1 monoclonal antobody, clone 5G7 (Cat # MAB10297) (blue) and negative control (red).
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 3987
  • Gene Name:
  • LIMS1
  • Gene Alias:
  • PINCH,PINCH1
  • Gene Description:
  • LIM and senescent cell antigen-like domains 1
  • Gene Summary:
  • The protein encoded by this gene is an adaptor protein which contains five LIM domains, or double zinc fingers. The protein is likely involved in integrin signaling through its LIM domain-mediated interaction with integrin-linked kinase, found in focal adhesion plaques. It is also thought to act as a bridge linking integrin-linked kinase to NCK adaptor protein 2, which is involved in growth factor receptor kinase signaling pathways. Its localization to the periphery of spreading cells also suggests that this protein may play a role in integrin-mediated cell adhesion or spreading. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000161608
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