LIMS1 monoclonal antibody, clone 5G7
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant LIMS1.
Immunogen
Recombinant protein corresponding to human LIMS1.
Host
Mouse
Theoretical MW (kDa)
37
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunofluorescence (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis using LIMS1 monoclonal antobody, clone 5G7 (Cat # MAB10297) against A-549 (1), Jurkat (2), and HeLa (3) cell lysate.Western Blot (Recombinant protein)
Western blot analysis using LIMS1 monoclonal antobody, clone 5G7 (Cat # MAB10297) against recombinant human LIMS1 protein.Immunofluorescence
Immunofluorescence analysis of HepG2 cells using LIMS1 monoclonal antobody, clone 5G7 (Cat # MAB10297) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of HeLa cells using LIMS1 monoclonal antobody, clone 5G7 (Cat # MAB10297) (blue) and negative control (red). -
Gene Info — LIMS1
Entrez GeneID
3987Gene Name
LIMS1
Gene Alias
PINCH, PINCH1
Gene Description
LIM and senescent cell antigen-like domains 1
Omim ID
602567Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is an adaptor protein which contains five LIM domains, or double zinc fingers. The protein is likely involved in integrin signaling through its LIM domain-mediated interaction with integrin-linked kinase, found in focal adhesion plaques. It is also thought to act as a bridge linking integrin-linked kinase to NCK adaptor protein 2, which is involved in growth factor receptor kinase signaling pathways. Its localization to the periphery of spreading cells also suggests that this protein may play a role in integrin-mediated cell adhesion or spreading. [provided by RefSeq
Other Designations
OTTHUMP00000161608
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