Mouse monoclonal antibody raised against recombinant PHB.
Recombinant protein corresponding to human PHB.
Theoretical MW (kDa):
Human, Monkey, Mouse, Rat
ELISA (1:10000) Western Blot (1:500-1:2000) Immunohistochemistry (1:200-1:1000) Immunofluorescence (1:200-1:1000) Flow cytometry (1:200-1:400) The optimal working dilution should be determined by the end user.
In ascites (0.03% sodium azide)
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue (A), liver cancer tissue (B), lung cancer tissue (C) and stomach cancer tissue (D) using PHB monoclonal antobody, clone 5H7 (Cat # MAB10296) with DAB staining.
Immunofluorescence analysis of NIH/3T3 cells using PHB monoclonal antobody, clone 5H7 (Cat # MAB10296) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow cytometric analysis of MCF-7 cells using PHB monoclonal antobody, clone 5H7 (Cat # MAB10296) (blue) and negative control (red).
Prohibitin is an evolutionarily conserved gene that is ubiquitously expressed. It is thought to be a negative regulator of cell proliferation and may be a tumor suppressor. Mutations in PHB have been linked to sporadic breast cancer. Prohibitin is expressed as two transcripts with varying lengths of 3' untranslated region. The longer transcript is present at higher levels in proliferating tissues and cells, suggesting that this longer 3' untranslated region may function as a trans-acting regulatory RNA. [provided by RefSeq