OLIG2 monoclonal antibody, clone 1G11
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis using OLIG2 monoclonal antobody, clone 1G11 (Cat # MAB10287) against HEK293 (1) and OLIG2-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human muscle tissue using OLIG2 monoclonal antobody, clone 1G11 (Cat # MAB10287) with DAB staining.
Immunofluorescence
Immunofluorescence analysis of U251 cells using OLIG2 monoclonal antobody, clone 1G11 (Cat # MAB10287) (green). Blue: DRAQ5 fluorescent DNA dye.
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant OLIG2.
Immunogen
Recombinant protein corresponding to human OLIG2.
Host
Mouse
Theoretical MW (kDa)
32
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunofluorescence (1:200-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis using OLIG2 monoclonal antobody, clone 1G11 (Cat # MAB10287) against HEK293 (1) and OLIG2-hIgGFc transfected HEK293 (2) cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human muscle tissue using OLIG2 monoclonal antobody, clone 1G11 (Cat # MAB10287) with DAB staining.Immunofluorescence
Immunofluorescence analysis of U251 cells using OLIG2 monoclonal antobody, clone 1G11 (Cat # MAB10287) (green). Blue: DRAQ5 fluorescent DNA dye.Enzyme-linked Immunoabsorbent Assay
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Gene Info — OLIG2
Entrez GeneID
10215Gene Name
OLIG2
Gene Alias
BHLHB1, OLIGO2, PRKCBP2, RACK17, bHLHe19
Gene Description
oligodendrocyte lineage transcription factor 2
Omim ID
606386Gene Ontology
HyperlinkGene Summary
This gene encodes a basic helix-loop-helix transcription factor which is expressed in oligodendroglial tumors of the brain. The protein is an essential regulator of ventral neuroectodermal progenitor cell fate. The gene is involved in a chromosomal translocation t(14;21)(q11.2;q22) associated with T-cell acute lymphoblastic leukemia. Its chromosomal location is within a region of chromosome 21 which has been suggested to play a role in learning deficits associated with Down syndrome. [provided by RefSeq
Other Designations
OTTHUMP00000067569|OTTHUMP00000067570|basic domain, helix-loop-helix protein, class B, 1|human protein kinase C-binding protein RACK17|oligodendrocyte-specific bHLH transcription factor 2|protein kinase C binding protein 2
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Interactome
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Disease
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