Il27 monoclonal antibody, clone 3H12.F10
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More Files
- More Functions
Western Blot (Recombinant protein)
Western blot analysis of recombinant protein with Il27 monoclonal antibody, clone 3H12.F10 (Cat # MAB10274) at 1:1000 dilution.
Flow Cytometry
Flow cytometric analysis of mouse peritoneal macrophages using Il27 monoclonal antibody, clone 3H12.F10 (Cat ID # MAB10274). Mouse peritoneal macrophages were grown in culture for 24 hours, stimulated with 10 ng/mL IFNa and 1 ug/mL LPS for 14 hours and incubated for 4 hours with Bredfeldin A. Cells were harvested, washed, aliquoted 1x106 cells per sample, and fixed and permeabilized according to a standard protocol. Samples were stained with biotinylated primary anti-mouse p28 antibody at 0.1-10 ug/mL primary antibody alongside negative controls of unstimulated cells and isotype controls. Cells were stained with 0.25 ug/mL Il27 monoclonal antibody, clone 3H12.F10 (Cat ID # MAB10274) and PHYCOERYTHRIN Conjugated secondary at 1:100 and analyzed by flow cytometry. Stimulated cells showed increase PE staining (horizontal axis) when compared with unstimulated cells.
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Specification
Product Description
Rat monoclonal antibody raised against recombinant Il27.
Immunogen
Recombinant protein corresponding to mouse Il27.
Host
Rat
Theoretical MW (kDa)
26
Reactivity
Mouse
Specificity
This antibody is specific for mouse and rat p28 protein. Cross-reactivity with IL-27 from other sources has not been determined.
Form
Liquid
Recommend Usage
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Recombinant protein)
Western blot analysis of recombinant protein with Il27 monoclonal antibody, clone 3H12.F10 (Cat # MAB10274) at 1:1000 dilution.Flow Cytometry
Flow cytometric analysis of mouse peritoneal macrophages using Il27 monoclonal antibody, clone 3H12.F10 (Cat ID # MAB10274). Mouse peritoneal macrophages were grown in culture for 24 hours, stimulated with 10 ng/mL IFNa and 1 ug/mL LPS for 14 hours and incubated for 4 hours with Bredfeldin A. Cells were harvested, washed, aliquoted 1x106 cells per sample, and fixed and permeabilized according to a standard protocol. Samples were stained with biotinylated primary anti-mouse p28 antibody at 0.1-10 ug/mL primary antibody alongside negative controls of unstimulated cells and isotype controls. Cells were stained with 0.25 ug/mL Il27 monoclonal antibody, clone 3H12.F10 (Cat ID # MAB10274) and PHYCOERYTHRIN Conjugated secondary at 1:100 and analyzed by flow cytometry. Stimulated cells showed increase PE staining (horizontal axis) when compared with unstimulated cells. -
Gene Info — Il27
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