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Last updated: 2016/11/27
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Il27 monoclonal antibody, clone 3H12.F10

  • Catalog # : MAB10274
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  • Specification
  • Product Description:
  • Rat monoclonal antibody raised against recombinant Il27.
  • Immunogen:
  • Recombinant protein corresponding to mouse Il27.
  • Host:
  • Rat
  • Theoretical MW (kDa):
  • 26
  • Reactivity:
  • Mouse
  • Specificity:
  • This antibody is specific for mouse and rat p28 protein. Cross-reactivity with IL-27 from other sources has not been determined.
  • Form:
  • Liquid
  • Recommend Usage:
  • Western Blot (1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • Western blot analysis of recombinant protein with Il27 monoclonal antibody, clone 3H12.F10 (Cat # MAB10274) at 1:1000 dilution.
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of mouse peritoneal macrophages using Il27 monoclonal antibody, clone 3H12.F10 (Cat ID # MAB10274). Mouse peritoneal macrophages were grown in culture for 24 hours, stimulated with 10 ng/mL IFNa and 1 ug/mL LPS for 14 hours and incubated for 4 hours with Bredfeldin A. Cells were harvested, washed, aliquoted 1x106 cells per sample, and fixed and permeabilized according to a standard protocol. Samples were stained with biotinylated primary anti-mouse p28 antibody at 0.1-10 ug/mL primary antibody alongside negative controls of unstimulated cells and isotype controls. Cells were stained with 0.25 ug/mL Il27 monoclonal antibody, clone 3H12.F10 (Cat ID # MAB10274) and PHYCOERYTHRIN Conjugated secondary at 1:100 and analyzed by flow cytometry. Stimulated cells showed increase PE staining (horizontal axis) when compared with unstimulated cells.
  • Application Image
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • enlarge
  • Gene Information
  • Gene Name:
  • Il27
  • Gene Alias:
  • IL-27,IL-27p28,Il30,p28
  • Gene Description:
  • interleukin 27
  • Other Designations:
  • IL-27 p28 subunit,interleukin 30
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