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Last updated: 2017/8/20
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Il17a monoclonal antibody, clone 20B4.G10.F5

  • Catalog # : MAB10273
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  • Specification
  • Product Description:
  • Rat monoclonal antibody raised against full length recombinant Il17a.
  • Immunogen:
  • Recombinant protein corresponding to full length mouse Il17a.
  • Host:
  • Rat
  • Theoretical MW (kDa):
  • 23
  • Reactivity:
  • Mouse
  • Specificity:
  • This antibody is specific for mouse IL-17A protein. Cross-reactivity with IL-17A from other sources has not been determined.
  • Form:
  • Liquid
  • Recommend Usage:
  • Western Blot (1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • Western blot analysis of recombinant protein with Il17a monoclonal antibody, clone 20B4.G10.F5 (Cat # MAB10273) at 1:1000 dilution.
  • Flow Cytometry
  • Flow Cytometry
  • Mouse CD4+ T cells were isolated from freshly dissected spleen by centrifugation in T cell separation media and selected by magnetic separation. Cells were grown on plates coated with anti-CD3 antibody, and stimulated with: 5 ug/mL anti-CD28, 10 ng/mL IL-1beta, 50 ng/mL mouse IL-6, 1 ng/mL TGFbeta1 and 10 ug/mL anti-mouse IFNa over 8-10 days of culture. Cells were incubated for 15-20 minutes with Add rat anti-mouse CD4 APC at a concentration of 0.125 ug/mL, washed, fixed and permeabilized and incubated with Il17a monoclonal antibody, clone 20B4.G10.F5 (Cat ID # MAB10273) or controls as shown. Cells were washed, incubated in streptavidin conjugated PE, fixed and analyzed by Flow cytometry.
  • Application Image
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • enlarge
  • Gene Information
  • Gene Name:
  • Il17a
  • Gene Alias:
  • Ctla-8,Ctla8,Il17
  • Gene Description:
  • interleukin 17A
  • Other Designations:
  • interleukin 17
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