EPCAM monoclonal antibody, clone VU-1D9
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More Files
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Immunofluorescence (Circulating Tumor Cell)
MCF-7 cells spiked whole blood sample flow through Captor®. Spiked MCF-7 cells were captured (Upper: Bright Field) and on-chip stained with EpCAM-FITC labeled monoclonal antibody (Lower: Green). The cell nucleus were counterstained with DAPI (Lower: Blue).
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Specification
Product Description
Mouse monoclonal antibody raised against EPCAM.
Immunogen
Human small cell lung carcinoma cell line H69.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Isotype
IgG1
Recommend Usage
Flow Cytometry (1-3 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (5-10 ug/mL)
Immunoprecipitation (1-2 ug/100-500 ug of protein in 1 mL lysate)
Western Blot (1-4 ug/mL)
Immunohistochemistry (Frozen sections) (4 ug/mL)
Immunocytochemistry (4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (0.09% sodium azide)
Storage Instruction
Store at 4°C. Do not freeze.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunohistochemistry
Immunocytochemistry
Immunoprecipitation
Flow Cytometry
Immunofluorescence (Circulating Tumor Cell)
MCF-7 cells spiked whole blood sample flow through Captor®. Spiked MCF-7 cells were captured (Upper: Bright Field) and on-chip stained with EpCAM-FITC labeled monoclonal antibody (Lower: Green). The cell nucleus were counterstained with DAPI (Lower: Blue). -
Gene Info — EPCAM
Entrez GeneID
4072Gene Name
EPCAM
Gene Alias
17-1A, 323/A3, CD326, CO-17A, CO17-1A, EGP, EGP-2, EGP34, EGP40, ESA, Ep-CAM, GA733-2, HEA125, KS1/4, KSA, M4S1, MH99, MIC18, MK-1, MOC31, TACST-1, TACSTD1, TROP1, hEGP-2
Gene Description
epithelial cell adhesion molecule
Omim ID
185535Gene Ontology
HyperlinkGene Summary
This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq
Other Designations
adenocarcinoma-associated antigen|carcinoma-associated antigen GA733-2|human epithelial glycoprotein-2|membrane component, chromosome 4, surface marker (35kD glycoprotein)|tumor-associated calcium signal transducer 1
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Interactome
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Disease
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