DYKDDDDK tag monoclonal antibody
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Specification
Product Description
Mouse monoclonal antibody raised against DYKDDDDK tag.
Immunogen
FLAG (DYKDDDDK) conjugated proteins.
Host
Mouse
Specificity
Recognize FLAG (DYKDDDDK) conjugated at either the amino or carboxyl terminus. Can be used to bound to various resins for affinity purifications.
Form
Liquid
Purification
Protein A/Ion exchange chromatography purification
Isotype
IgG1, kappa
Recommend Usage
ELISA
Immunofluorescence
Immunoprecipitation
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In non-hazardous buffer.
Storage Instruction
Store at 4°C for short term. For long term storage, store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Transfected lysate)
Western Blot analysis of Flag (DYKDDDDK) expression in transfected 293T cell line with DYKDDDDK tag monoclonal antibody (Cat # MAB10142).Immunofluorescence
Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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