ADK monoclonal antibody, clone AT4F8
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of cell extracts (35 ug) with ADK monoclonal antibody, clone AT4F8 (Cat # MAB10016) at 1:1000 dilution.
Lane 1, HepG2.
Lane 2, 293T.
Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Immunofluorescence
Immunofluorescence analysis of A-549 cells. The cell was stained with ADK monoclonal antibody, clone AT4F8 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Flow Cytometry
Flow cytometry analysis of A-549 cell line, staining at 2-5 ug for 1x106cell (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant ADK.
Immunogen
Recombinant protein corresponding to amino acids 22-362 of human ADK.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Concentration
1 mg/mL
Isotype
IgG1, kappa
Recommend Usage
ELISA
Flow Cytometry
Immunocytochemistry
Immunofluorescence
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of cell extracts (35 ug) with ADK monoclonal antibody, clone AT4F8 (Cat # MAB10016) at 1:1000 dilution.
Lane 1, HepG2.
Lane 2, 293T.
Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Immunocytochemistry
Immunofluorescence
Immunofluorescence analysis of A-549 cells. The cell was stained with ADK monoclonal antibody, clone AT4F8 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometry analysis of A-549 cell line, staining at 2-5 ug for 1x106cell (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line). -
Gene Info — ADK
Entrez GeneID
132Protein Accession#
NP_006712Gene Name
ADK
Gene Alias
AK
Gene Description
adenosine kinase
Omim ID
102750Gene Ontology
HyperlinkGene Summary
This gene encodes adenosine kinase, an abundant enzyme in mammalian tissues. The enzyme catalyzes the transfer of the gamma-phosphate from ATP to adenosine, thereby serving as a regulator of concentrations of both extracellular adenosine and intracellular adenine nucleotides. Adenosine has widespread effects on the cardiovascular, nervous, respiratory, and immune systems and inhibitors of the enzyme could play an important pharmacological role in increasing intravascular adenosine concentrations and acting as anti-inflammatory agents. Alternative splicing results in two transcript variants encoding different isoforms. Both isoforms of the enzyme phosphorylate adenosine with identical kinetics and both require Mg2+ for activity. [provided by RefSeq
Other Designations
OTTHUMP00000019864|OTTHUMP00000019865|adenosine 5'-phosphotransferase
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Interactome
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Pathway
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Disease
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