Product Browser

Last updated: 2021/1/17

Product Compare

Product Compare Cancel Click this icon to add products to compare list. Select up to 10 products.

Quick Order (Tutorial)

Input Catalog #,
place order here!
Catalog # :
  • Where to buy
  • Choose your location
Bulk order
Custom Services
2019 Next Day Shipping

ADK monoclonal antibody, clone AT4F8 

  • Catalog # : MAB10016
  • Visit Frequency :
  • Countries :
  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant ADK.
  • Immunogen:
  • Recombinant protein corresponding to amino acids 22-362 of human ADK.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein G purification
  • Concentration:
  • 1 mg/mL
  • Isotype:
  • IgG1, kappa
  • Recommend Usage:
  • ELISA
    Flow Cytometry
    Immunocytochemistry
    Immunofluorescence
    Western Blot
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
  • Storage Instruction:
  • Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of cell extracts (35 ug) with ADK monoclonal antibody, clone AT4F8 (Cat # MAB10016) at 1:1000 dilution.
    Lane 1, HepG2.
    Lane 2, 293T.
    Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
  • Immunocytochemistry
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence analysis of A-549 cells. The cell was stained with ADK monoclonal antibody, clone AT4F8 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
  • Enzyme-linked Immunoabsorbent Assay
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometry analysis of A-549 cell line, staining at 2-5 ug for 1x106cell (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunocytochemistry
  • Enzyme-linked Immunoabsorbent Assay
  • Gene Information
  • Entrez GeneID:
  • 132
  • Gene Name:
  • ADK
  • Gene Alias:
  • AK
  • Gene Description:
  • adenosine kinase
  • Gene Summary:
  • This gene encodes adenosine kinase, an abundant enzyme in mammalian tissues. The enzyme catalyzes the transfer of the gamma-phosphate from ATP to adenosine, thereby serving as a regulator of concentrations of both extracellular adenosine and intracellular adenine nucleotides. Adenosine has widespread effects on the cardiovascular, nervous, respiratory, and immune systems and inhibitors of the enzyme could play an important pharmacological role in increasing intravascular adenosine concentrations and acting as anti-inflammatory agents. Alternative splicing results in two transcript variants encoding different isoforms. Both isoforms of the enzyme phosphorylate adenosine with identical kinetics and both require Mg2+ for activity. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000019864,OTTHUMP00000019865,adenosine 5'-phosphotransferase
  • RSS
  • YouTube
  • Linkedin
  • Facebook