Western blot analysis of cell extracts (35 ug) with ADK monoclonal antibody, clone AT4F8 (Cat # MAB10016) at 1:1000 dilution. Lane 1, HepG2. Lane 2, 293T. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Immunocytochemistry
Immunofluorescence
Immunofluorescence analysis of A-549 cells. The cell was stained with ADK monoclonal antibody, clone AT4F8 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometry analysis of A-549 cell line, staining at 2-5 ug for 1x106cell (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).
This gene encodes adenosine kinase, an abundant enzyme in mammalian tissues. The enzyme catalyzes the transfer of the gamma-phosphate from ATP to adenosine, thereby serving as a regulator of concentrations of both extracellular adenosine and intracellular adenine nucleotides. Adenosine has widespread effects on the cardiovascular, nervous, respiratory, and immune systems and inhibitors of the enzyme could play an important pharmacological role in increasing intravascular adenosine concentrations and acting as anti-inflammatory agents. Alternative splicing results in two transcript variants encoding different isoforms. Both isoforms of the enzyme phosphorylate adenosine with identical kinetics and both require Mg2+ for activity. [provided by RefSeq