NELL2 monoclonal antibody, clone AT13E7
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot analysis of mouse brain tissue extracts with NELL2 monoclonal antibody, clone AT13E7 (Cat # MAB10014) at 1:500 dilution. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Western Blot (Cell lysate)
Western blot analysis of cell extracts with NELL2 monoclonal antibody, clone AT13E7 (Cat # MAB10014) at 1:500 dilution.
Lane 1, HeLa.
Lane 2, AGS.
Lane 3, NIH/3T3.
Lane 4, 293T.
Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant NELL2.
Immunogen
Recombinant protein corresponding to amino acids 30-258 of human NELL2.
Host
Mouse
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein G purification
Concentration
1 mg/mL
Isotype
IgG2b, kappa
Recommend Usage
ELISA
Western Blot (1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of mouse brain tissue extracts with NELL2 monoclonal antibody, clone AT13E7 (Cat # MAB10014) at 1:500 dilution. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Western Blot (Cell lysate)
Western blot analysis of cell extracts with NELL2 monoclonal antibody, clone AT13E7 (Cat # MAB10014) at 1:500 dilution.
Lane 1, HeLa.
Lane 2, AGS.
Lane 3, NIH/3T3.
Lane 4, 293T.
Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Enzyme-linked Immunoabsorbent Assay
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Gene Info — NELL2
Entrez GeneID
4753Protein Accession#
NP_006150Gene Name
NELL2
Gene Alias
NRP2
Gene Description
NEL-like 2 (chicken)
Omim ID
602320Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a glycoprotein containing several von Willebrand factor C domains and epidermal growth factor (EGF)-like domains. The encoded protein acts as a homotrimer and is found in the cytoplasm. Several variants encoding a few different isoforms exist, and at least one isoform appears to be a secreted protein. Studies in mouse suggest that this protein plays a role in neural cell growth and differentiation as well as in oncogenesis. [provided by RefSeq
Other Designations
nel-like 2|neural epidermal growth factor-like 2
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Interactome
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Disease
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