Immunoprecipitation (Lane 1 and 2) and Western blot (Lane 3, 4 and 5) analysis in various cells and tissue extracts with AXIN1 monoclonal antibody, clone AT1A4 (Cat # MAB10012) at 1:500 dilution. Lane 1, normal IgG. Lane 2, HeLa. Lane 3, HeLa. Lane 4, AGS. Lane 5, SW480.
Immunofluorescence staining of human HeLa cells was done with Hoechst 33342 (Blue) for chromatin staining and Texas Red (Red) with AXIN1 monoclonal antibody, clone AT1A4 (Cat # MAB10012) at 1:500 dilution.
This gene encodes a cytoplasmic protein which contains a regulation of G-protein signaling (RGS) domain and a dishevelled and axin (DIX) domain. The encoded protein interacts with adenomatosis polyposis coli, catenin (cadherin-associated protein), beta 1, 88kDa, glycogen synthase kinase 3 beta, protein phosphate 2, and itself. This protein functions as a negative regulator of the wingless-type MMTV integration site family, member 1 (WNT) signaling pathway and can induce apoptosis. The crystal structure of a portion of this protein, alone and in a complex with other proteins, has been resolved. Mutations in this gene have been associated with hepatocellular carcinoma, hepatoblastomas, ovarian endometriod adenocarcinomas, and medullablastomas. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq
axis inhibition protein 1,axis inhibitor 1,fused, mouse, homolog of