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ARF1 monoclonal antibody, clone AT1B3

  • Catalog # : MAB10011
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against full length recombinant ARF1.
  • Immunogen:
  • Recombinant protein corresponding to full length human ARF1.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein G purification
  • Concentration:
  • 1 mg/mL
  • Isotype:
  • IgG1, kappa
  • Recommend Usage:
  • ELISA
    Flow Cytometry (2.5 ug for 106 cells)
    Immunocytochemistry (1:100)
    Immunofluorescence (1:100)
    Western Blot (1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot
  • Western Blot
  • Western Blot analysis of (1) HeLa cell lysate, (2) HepG2 cell lysate, (3) brain tissue lysate and (4) MCF cell lysate.
  • Immunocytochemistry
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescent staining of MCF-7 cell line stained with DAPI (Blue) for nucleus staining and ARF1 monoclonal antibody, clone AT1B3 (Cat # MAB10011) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescent staining of U87 MG cell line stained with DAPI (Blue) for nucleus staining and ARF1 monoclonal antibody, clone AT1B3 (Cat # MAB10011) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
  • ELISA
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of ARF1 in MCF-7 cell line, staining at 2-5 ug for 1 x 106 cells (Red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (Black line).
  • Application Image
  • Immunocytochemistry
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 375
  • Gene Name:
  • ARF1
  • Gene Alias:
  • -
  • Gene Description:
  • ADP-ribosylation factor 1
  • Gene Summary:
  • ADP-ribosylation factor 1 (ARF1) is a member of the human ARF gene family. The family members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking as activators of phospholipase D. The gene products, including 6 ARF proteins and 11 ARF-like proteins, constitute a family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2 and ARF3), class II (ARF4 and ARF5) and class III (ARF6), and members of each class share a common gene organization. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000035715
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