ARF1 monoclonal antibody, clone AT1B3
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More Files
- More Functions
Western Blot
Western Blot analysis of (1) HeLa cell lysate, (2) HepG2 cell lysate, (3) brain tissue lysate and (4) MCF cell lysate.
Immunofluorescence
Immunofluorescent staining of U87 MG cell line stained with DAPI (Blue) for nucleus staining and ARF1 monoclonal antibody, clone AT1B3 (Cat # MAB10011) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Immunofluorescence
Immunofluorescent staining of MCF-7 cell line stained with DAPI (Blue) for nucleus staining and ARF1 monoclonal antibody, clone AT1B3 (Cat # MAB10011) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Flow Cytometry
Flow cytometric analysis of ARF1 in MCF-7 cell line, staining at 2-5 ug for 1 x 106 cells (Red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (Black line).
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Specification
Product Description
Mouse monoclonal antibody raised against full length recombinant ARF1.
Immunogen
Recombinant protein corresponding to full length human ARF1.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Concentration
1 mg/mL
Isotype
IgG1, kappa
Recommend Usage
ELISA
Flow Cytometry (2.5 ug for 106 cells)
Immunocytochemistry (1:100)
Immunofluorescence (1:100)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of (1) HeLa cell lysate, (2) HepG2 cell lysate, (3) brain tissue lysate and (4) MCF cell lysate.Immunocytochemistry
Immunofluorescence
Immunofluorescent staining of U87 MG cell line stained with DAPI (Blue) for nucleus staining and ARF1 monoclonal antibody, clone AT1B3 (Cat # MAB10011) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).Immunofluorescence
Immunofluorescent staining of MCF-7 cell line stained with DAPI (Blue) for nucleus staining and ARF1 monoclonal antibody, clone AT1B3 (Cat # MAB10011) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of ARF1 in MCF-7 cell line, staining at 2-5 ug for 1 x 106 cells (Red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (Black line). -
Gene Info — ARF1
Entrez GeneID
375Protein Accession#
NP_ 001649Gene Name
ARF1
Gene Alias
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Gene Description
ADP-ribosylation factor 1
Omim ID
103180Gene Ontology
HyperlinkGene Summary
ADP-ribosylation factor 1 (ARF1) is a member of the human ARF gene family. The family members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking as activators of phospholipase D. The gene products, including 6 ARF proteins and 11 ARF-like proteins, constitute a family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2 and ARF3), class II (ARF4 and ARF5) and class III (ARF6), and members of each class share a common gene organization. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000035715
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Interactome
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Pathway
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Disease
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Publication Reference
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Arf1 coordinates fatty acid metabolism and mitochondrial homeostasis.
Ludovic Enkler, Viktoria Szentgyörgyi, Mirjam Pennauer, Cristina Prescianotto-Baschong, Isabelle Riezman, Aneta Wiesyk, Reut Ester Avraham, Martin Spiess, Einat Zalckvar, Roza Kucharczyk, Howard Riezman, Anne Spang.
Nature Cell Biology 2023 Aug; 25(8):1157.
Application：WB, Human, Yeast, HeLa cells, Saccharomyces cerevisiae.
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Arf1 coordinates fatty acid metabolism and mitochondrial homeostasis.
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