NRAS monoclonal antibody, clone AT2G9
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of 293T cell extracts (35 ug) with NRAS monoclonal antibody, clone AT2G9 (Cat # MAB10009) at 1:500 (Lane 1), 1:1000 (Lane 2) and 1:5000 (Lane 3) dilution. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Western Blot (Recombinant protein)
Western blot analysis with recombinant protein (200 ng) of NRAS (Lane 1), KRAS (Lane 2) and HRAS (Lane 3). Using NRAS monoclonal antibody, clone AT2G9 (Cat # MAB10009).
-
Specification
Product Description
Mouse monoclonal antibody raised against full length recombinant NRAS.
Immunogen
Recombinant protein corresponding to full length human NRAS.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Concentration
1 mg/mL
Isotype
IgG2a, kappa
Recommend Usage
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Cell lysate)
Western blot analysis of 293T cell extracts (35 ug) with NRAS monoclonal antibody, clone AT2G9 (Cat # MAB10009) at 1:500 (Lane 1), 1:1000 (Lane 2) and 1:5000 (Lane 3) dilution. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Western Blot (Recombinant protein)
Western blot analysis with recombinant protein (200 ng) of NRAS (Lane 1), KRAS (Lane 2) and HRAS (Lane 3). Using NRAS monoclonal antibody, clone AT2G9 (Cat # MAB10009).Enzyme-linked Immunoabsorbent Assay
-
Gene Info — NRAS
Entrez GeneID
4893Protein Accession#
NP_002515Gene Name
NRAS
Gene Alias
ALPS4, N-ras, NRAS1
Gene Description
neuroblastoma RAS viral (v-ras) oncogene homolog
Gene Ontology
HyperlinkGene Summary
This is an N-ras oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. The encoded protein, which has intrinsic GTPase activity, is activated to a GTP-bound form by a GTPase activating protein and inactivated to a GDP-bound form by a guanine nucleotide-exchange factor. Defects in this gene are a cause of juvenile myelomonocytic leukemia (JMML). [provided by RefSeq
Other Designations
N-ras protein part 4|OTTHUMP00000013879|v-ras neuroblastoma RAS viral oncogene homolog
-
Interactome
-
Pathway
-
Disease
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com