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Last updated: 2018/1/14
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Histone H2B (ubiquitinated) monoclonal antibody, clone NRO3

  • Catalog # : MAB10006
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against synthetic peptide of Histone H2B (ubiquitinated).
  • Immunogen:
  • A branched peptide corresponding to the conjugation site of ubiquitin on human Histone H2B.
  • Host:
  • Mouse
  • Reactivity:
  • Arabidopsis, Fruit fly, Human, Mouse
  • Specificity:
  • The antibody recognizes the K120 ubiquitination of the human histone H2B with no detectable cross-reactivity with either non-ubiquitinated H2B or ubiquitinated.
  • Form:
  • Lyophilized
  • Purification:
  • Protein G purification
  • Isotype:
  • IgG2a
  • Recommend Usage:
  • Immunohistochemistry (1:200-500)
    Western Blot (2-3 ug/mL)
    ChIP (3-5 ug/CHIP)
    Immunofluorescence
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • Lyophilized from 2 ug/ul solution in PBS, pH 7.4.
  • Storage Instruction:
  • Store at 4°C for three months. For long term storage store at -20°C.
    After reconstitution with distilled water, store at -20°C or lower.
    Aliquot to avoid repeated freezing and thawing.
  • Applications
  • ChIP
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western Blot (Cell lysate) analysis of (1) HeLa cells transfected with siRNA directed against LacZ, (2) siRNA directed against the H2B E3 ubiquitin ligase (hBRE1).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunofluorescence
  • Application Image
  • ChIP
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunofluorescence
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