Mouse monoclonal antibody raised against synthetic peptide of HLA-G.
A synthetic peptide (conjugated with OVA) corresponding to C-terminus of human soluble HLA-G5 and HLA-G6.
This antibody allows to discriminate between soluble HLA-G protein yielded by shedding from membrane-bound HLA-G forms that do not contain the intron 4-encoded epitope and soluble HLA-G5/HLA-G6 produced from intron 4-retaining alternatively spliced mRNAs.
The optimal working dilution should be determined by the end user.
In PBS, pH 7.4 (0.09% sodium azide)
Store at 4°C. Do not freeze. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western blotting analysis of whole cell lysate of HLA - G stable transfectants (various splice variants) using HLA - G monoclonal antibody, clone 5A6G7 (Cat # MAB0965) . Lane 1 : M8 cell line transfected with empty vector. Lane 2 : M8 cell line transfected with HLA - G1. Lane 3 : M8 cell line transfected with HLA - G5. Lane 4 : M8 cell line transfected with HLA - G6.
HLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exon 6 encodes the cytoplasmic tail. [provided by RefSeq
HLA class I molecule,HLA-G histocompatibility antigen, class I, G,MHC class I antigen,OTTHUMP00000039485,b2 microglobulin