FYN monoclonal antibody, clone FYN-01
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More Files
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Immunofluorescence
Immunofluorescence staining of FYN in human HeLa cell line using FYN monoclonal antibody, clone FYN - 01 (Cat # MAB0947 ; green) . Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue) .
Immunofluorescence
Immunofluorescence staining of FYN in murine transformed fibroblasts using FYN monoclonal antibody, clone FYN - 01 (Cat # MAB0947 ; red) . Actin cytoskeleton was decorated by phalloidin (green) and cell nuclei stained with DAPI (blue) .
Immunofluorescence
Immunofluorescence staining of FYN in human primary fibroblasts using FYN monoclonal antibody, clone FYN - 01 (Cat # MAB0947 ; green) . Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue) .
Immunoprecipitation
Immunoprecipitation of FYN from the lysate of T cells isolated from fresh buffy coats. Western blot was immunostained with FYN monoclonal antibody, clone FYN - 01 (Cat # MAB0947) . Lane 1 : Original lysate of T cells. Lane 2 - 3 : Immunoprecipitated material eluted from affinity sorbent (FYN - 01 coupled to Sepharose beads) . Lanes differ in amount of T cell lysate loaded on the immunosorbent.
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant FYN.
Immunogen
Recombinant protein corresponding to amino acids 7-176 of human FYN.
Host
Mouse
Reactivity
Human, Mouse
Form
Liquid
Concentration
1 mg/mL
Isotype
IgG
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In PBS, pH 7.4 (0.09% sodium azide)
Storage Instruction
Store at 4°C. Do not freeze.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunocytochemistry
Immunofluorescence
Immunofluorescence staining of FYN in human HeLa cell line using FYN monoclonal antibody, clone FYN - 01 (Cat # MAB0947 ; green) . Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue) .Immunofluorescence
Immunofluorescence staining of FYN in murine transformed fibroblasts using FYN monoclonal antibody, clone FYN - 01 (Cat # MAB0947 ; red) . Actin cytoskeleton was decorated by phalloidin (green) and cell nuclei stained with DAPI (blue) .Immunofluorescence
Immunofluorescence staining of FYN in human primary fibroblasts using FYN monoclonal antibody, clone FYN - 01 (Cat # MAB0947 ; green) . Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue) .Immunoprecipitation
Immunoprecipitation of FYN from the lysate of T cells isolated from fresh buffy coats. Western blot was immunostained with FYN monoclonal antibody, clone FYN - 01 (Cat # MAB0947) . Lane 1 : Original lysate of T cells. Lane 2 - 3 : Immunoprecipitated material eluted from affinity sorbent (FYN - 01 coupled to Sepharose beads) . Lanes differ in amount of T cell lysate loaded on the immunosorbent. -
Gene Info — FYN
Entrez GeneID
2534Gene Name
FYN
Gene Alias
MGC45350, SLK, SYN
Gene Description
FYN oncogene related to SRC, FGR, YES
Omim ID
137025Gene Ontology
HyperlinkGene Summary
This gene is a member of the protein-tyrosine kinase oncogene family. It encodes a membrane-associated tyrosine kinase that has been implicated in the control of cell growth. The protein associates with the p85 subunit of phosphatidylinositol 3-kinase and interacts with the fyn-binding protein. Alternatively spliced transcript variants encoding distinct isoforms exist. [provided by RefSeq
Other Designations
OKT3-induced calcium influx regulator|OTTHUMP00000017914|OTTHUMP00000017915|OTTHUMP00000017917|c-syn protooncogene|protein-tyrosine kinase fyn|proto-oncogene tyrosine-protein kinase fyn|src-like kinase|src/yes-related novel|tyrosine kinase p59fyn(T)
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Interactome
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Pathway
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Disease
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