Immunofluorescence staining of DAXX in transfected HeLa human cervix carcinoma cell line. DAXX (green) was stained with DAXX monoclonal antibody, clone DAXX - 01 (Cat # MAB0937) , nuclei were stained with DAPI (blue) . A : Nuclear localization of DAXX in HeLa cells transfected with pCDNA3 - MycDaxx. B : HeLa cells were co - transfected with pCDNA3 - MycDaxx and pCDNA3 - ASK1HA, which led to translocation of DAXX from nucleus to cytoplasm.
This gene encodes a multifunctional protein that resides in multiple locations in the nucleus and in the cytoplasm. It interacts with a wide variety of proteins, such as apoptosis antigen Fas, centromere protein C, and transcription factor erythroblastosis virus E26 oncogene homolog 1. In the nucleus, the encoded protein functions as a potent transcription repressor that binds to sumoylated transcription factors. Its repression can be relieved by the sequestration of this protein into promyelocytic leukemia nuclear bodies or nucleoli. This protein also associates with centromeres in G2 phase. In the cytoplasm, the encoded protein may function to regulate apoptosis. The subcellular localization and function of this protein are modulated by post-translational modifications, including sumoylation, phosphorylation and polyubiquitination. Alternative splicing results in multiple transcript variants. [provided by RefSeq
CENP-C binding protein,ETS1-associated protein 1,Fas-binding protein,OTTHUMP00000029289,OTTHUMP00000029290,death-associated protein 6