Mouse monoclonal antibody raised against native ITGB2.
Native purified ITGB2 from peripheral blood mononuclear cells.
Theoretical MW (kDa):
This antibody MEM-148 recognizes the epitope exposed on free human CD18 chains unassociated with CD11 as well as on high-affinity state of LFA-1. CD18 (integrin beta2 subunit; beta2 integrin) is a 90-95 KDa type I transmembrane protein expressed on all leukocytes.
The optimal working dilution should be determined by the end user.
In PBS, pH 7.4 (0.09% sodium azide)
Store at 4°C. Do not freeze. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western blotting analysis of PMA - activated neutrophils (A) and monocytes (B) , using ITGB2 monoclonal antibody, clone MEM - 148 (Cat # MAB0929) . The antibody MEM - 148 recognizes the lower 65 to 70 kDa zone (activation marker) as well as the upper 78 to 96 kDa zone (ITGB2) .
The product of this gene belongs to the integrin beta chain family of proteins. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. This gene encodes the integrin beta chain beta 2. A given chain may combine with multiple partners resulting in different integrins. For example, beta 2 combines with the alpha L chain to form the integrin LFA-1, and combines with the alpha M chain to form the integrin Mac-1. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling. Defects in this gene are the cause of leukocyte adhesion deficiency type I (LAD1). Two transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq