Mouse monoclonal antibody raised against native CD27.
Immunogen:
Native purified CD27 from human peripheral blood lymphocytes.
Host:
Mouse
Theoretical MW (kDa):
50-55
Reactivity:
Human
Specificity:
This antibody reacts with CD27 (T14), a 50-55 KDa type I transmembrane glycoprotein (member of the TNF-receptor superfamily) expressed on medullary thymocytes, peripheral T lymphocytes, some B lymphocytes and NK cells.
Form:
Liquid
Purification:
Protein-A affinity chromatography.
Isotype:
IgG2a
Recommend Usage:
The optimal working dilution should be determined by the end user.
Storage Buffer:
In PBS, pH 7.4 (0.09% sodium azide)
Storage Instruction:
Store at 4°C. Do not freeze. Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Surface staining of human peripheral blood cells with CD27 monoclonal antibody, clone LT27 (Cat # MAB0923) PE. Cells in the lymphocyte gate were used for analysis.
The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor. [provided by RefSeq