Mouse monoclonal antibody raised against native TFRC.
Native purified TFRC from human pre-B cell line NALM-6.
Theoretical MW (kDa):
This antibody reacts with CD71 antigen (transferrin receptor), a 95 KDa type II homodimeric transmembrane glycoprotein expressed on activated B and lymphocytes, macrophages and erythroid precursors; it is lost on resting blood leukocytes. This antibody MEM-75 does not block binding of transferrin to the receptor.
The optimal working dilution should be determined by the end user.
In PBS, pH 7.4 (0.09% sodium azide)
Store at 4°C. Do not freeze. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Immunofluorescence staining of TFRC in human HeLa cell line using TFRC monoclonal antibody, clone MEM-75 (Cat # MAB0897 , green). Actin cytoskeleton decorated by phalloidin (red) and cell nuclei stained with DAPI (blue) .
Surface staining of NALM-6 human peripheral blood pre - B cell leukemia cell line with TFRC monoclonal antibody, clone MEM - 75 (Cat # MAB0897) biotin; detection by Streptavidin - PE. Total viable cells were used for analysis.