SPN monoclonal antibody, clone MEM-59
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Specification
Product Description
Mouse monoclonal antibody raised against native SPN.
Immunogen
Native purified SPN from human T lymphocytes.
Host
Mouse
Reactivity
Human
Specificity
This antibody recognizes neuraminidase-sensitive epitope on CD43 (Leukosialin), CD43 is expressed by platelets and at high levels on the surface of all leukocytes; it is negative on resting B lymphocytes and erythrocytes.
Form
Liquid
Isotype
IgG1
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In PBS, pH 7.4 (0.09% sodium azide)
Storage Instruction
Store at 4°C. Do not freeze.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blotting analysis (non - reducing conditions) of isolated peripheral blood lymphocytes of various species using SPN monoclonal antibody, clone MEM - 59 (Cat # MAB0884) . Lane 1 : Lysate of human PBL. Lane 2 : Lysate of canine PBL. Lane 3 : Lysate of porcine PBL.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoprecipitation
Flow Cytometry
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Gene Info — SPN
Entrez GeneID
6693Gene Name
SPN
Gene Alias
CD43, GPL115, LSN
Gene Description
sialophorin
Omim ID
182160Gene Ontology
HyperlinkGene Summary
Sialophorin (leukosialin) is a major sialoglycoprotein on the surface of human T lymphocytes, monocytes, granulocytes, and some B lymphocytes, which appears to be important for immune function and may be part of a physiologic ligand-receptor complex involved in T-cell activation.[supplied by OMIM
Other Designations
leukosialin|sialophorin (gpL115, leukosialin, CD43)|sialophorin (leukosialin, CD43)
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Interactome
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Pathway
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Disease
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