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Last updated: 2017/7/23
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MAPK3 (phospho T202/Y204) monoclonal antibody, clone 9B3

  • Catalog # : MAB0844
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against synthetic phosphopeptide of MAPK3.
  • Immunogen:
  • Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding T202/Y204 of human MAPK3.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Ammonium sulfate precipitation
  • Isotype:
  • IgG2b, kappa
  • Recommend Usage:
  • Western Blot (1:2000-1:5000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In HEPES, 150 mM NaCl (50% glycerol, 0.01% BSA, 0.03% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • MAPK3 (phospho T202/Y204) monoclonal antibody, clone 9B3 (Cat # MAB0844). Western blot analysis of MAPK3 expression in human kidney.
  • PDF DownloadProtocol Download
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • MAPK3 (phospho T202/Y204) monoclonal antibody, clone 9B3 (Cat # MAB0844). Western blot analysis of MAPK3 expression in HeLa.
  • PDF DownloadProtocol Download
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Immunoblot analysis of NIH/3T3 cell lysate stimulated with MAPK3 (phospho-T202/Y204) monoclonal antibody, clone 9B3 (Cat # MAB0844). (* N. S : Nonspecific band)
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Immunoblot analysis of cell lysates. Lane 1 : U87 MG cell lysate. Lane 2 : HepG2 cell lysate. Lane 3 : Jurkat cell lysate. Lane 4 : A-431 cell lysate.
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 5595
  • Gene Name:
  • MAPK3
  • Gene Alias:
  • ERK1,HS44KDAP,HUMKER1A,MGC20180,P44ERK1,P44MAPK,PRKM3
  • Gene Description:
  • mitogen-activated protein kinase 3
  • Gene Summary:
  • The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000174538,OTTHUMP00000174540,extracellular signal-regulated kinase 1,extracellular signal-related kinase 1
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