Product Browser

Last updated: 2020/8/9
  • Related Product Showcase

Product Compare

Product Compare Cancel Click this icon to add products to compare list. Select up to 10 products.

Quick Order (Tutorial)

Input Catalog #,
place order here!
Catalog # :
  • Where to buy
  • Choose your location

TP53 (phospho S315) monoclonal antibody, clone 18 

  • Catalog # : MAB0660
  • Visit Frequency :
  • Countries :
  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against synthetic phosphopeptide of TP53.
  • Immunogen:
  • Synthetic phosphopeptide corresponding to residues surrounding S315 of human TP53.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Isotype:
  • IgG2b, kappa
  • Recommend Usage:
  • ELISA
    Immunofluorescence
    Immunohistochemistry
    Western Blot (1:1000-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.4 (50% glycerol).
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • Upon arrival, centrifuge briefly.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Identification of TP53 (phospho-S315) monoclonal antibody, clone 18 (Cat # MAB0660) by Western blotting. Using crude cell extracts of MCF-7 untreated (lane 1) and treated with nocodazoleat 100 ng/ml for 48 h (lane2).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunoperoxidase of TP53 (phospho-S315) monoclonal antibody, clone 18 (Cat # MAB0660) on formalin-fixed paraffin-embedded human prostate.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunoperoxidase of TP53 (phospho-S315) monoclonal antibody, clone 18 (Cat # MAB0660) on formalin-fixed paraffin-embedded human stomach.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunoperoxidase of TP53 (phospho-S315) monoclonal antibody, clone 18 (Cat # MAB0660) on formalin-fixed paraffin-embedded human ovary.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence staining of p53 phosho-Ser315 in nuclei of HeLa cells subjected DNA damage.
    1. HeLa cells were treated with 100 nM Doxorubicin for 24 hr, fixed with 4% paraformaldehyde overnight, permealized with 0.25% Triton X-100 in PBS for 10 min. 2. Incubate cells with 1.5% BSA in PBS for 30 min to block unspecific binding of the antibodies. Incubate the cells with 1/1,000 diluted TP53 (phospho S315) monoclonal antibody, clone 18 (Cat # MAB0660) in 1% BSA in PBS at 4°C overnight. Incubate cells with the secondary antibody, goat anti-mouse IgG conjugated with Alex 488, at 1/1,000 dilution in 1% BSA for 1 hr at room temperature. 4. Nucleus (DNA) was stained with DAPI.
  • Enzyme-linked Immunoabsorbent Assay
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Enzyme-linked Immunoabsorbent Assay
  • Gene Information
  • Entrez GeneID:
  • 7157
  • Gene Name:
  • TP53
  • Gene Alias:
  • FLJ92943,LFS1,TRP53,p53
  • Gene Description:
  • tumor protein p53
  • Gene Summary:
  • This gene encodes tumor protein p53, which responds to diverse cellular stresses to regulate target genes that induce cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. p53 protein is expressed at low level in normal cells and at a high level in a variety of transformed cell lines, where it's believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing transcription activation, DNA-binding, and oligomerization domains. It is postulated to bind to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and hence cause the loss of tumor suppressor activity. Alterations of this gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families with Li-Fraumeni syndrome. Multiple p53 variants due to alternative promoters and multiple alternative splicing have been found. These variants encode distinct isoforms, which can regulate p53 transcriptional activity. [provided by RefSeq
  • Other Designations:
  • p53 antigen,p53 transformation suppressor,p53 tumor suppressor,phosphoprotein p53,transformation-related protein 53
  • Interactome
  • Interactome
  • Related Disease
  • RSS
  • YouTube
  • Linkedin
  • Facebook