Immunohistochemical staining of NGFR in Balb / C mouse brain (septum) using NGFR monoclonal antibody (Cat # MAB0291) . 4 % paraformaldehyde fixed mouse brain free floating sections were incubated with NGFR monoclonal antibody (1 ug / ml) overnight , followed by incubation with biotinylated goat anti -mouse IgG conjugate at a dilution of 1 : 500 and Vector ABC , DAB stained.
Immunohistochemistry (Frozen sections)
Immunofluorescent detection of NGFR in cultured mouse NSC-34 cells using a NGFR monoclonal antibody (Cat # MAB0291).
Immunofluorescence staining of adult mouse basal forebrain cholinergic neurons with NGFR monoclonal antibody (Cat # MAB0291), with ALEXA488 secondary antibodies.
Flow cytometry analysis of BSR-p75 fibroblasts transfected with mouse NGFR and endogenously expressed p75NTR analysis on NSC-34 motor neuron cells. X-63 conjugated control IgG represents negative control for all experiments.
Nerve growth factor receptor contains an extracellular domain containing four 40-amino acid repeats with 6 cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain, and a 155-amino acid cytoplasmic domain. The cysteine-rich region contains the nerve growth factor binding domain. [provided by RefSeq