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Last updated: 2017/9/17
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NGFR monoclonal antibody, clone MLR2

  • Catalog # : MAB0291
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant NGFR.
  • Immunogen:
  • Recombinant protein corresponding to extracellular region of human NGFR.
  • Host:
  • Mouse
  • Reactivity:
  • Human, Mouse
  • Form:
  • Lyophilized
  • Purification:
  • Protein G purification
  • Isotype:
  • IgG2a
  • Recommend Usage:
  • Immunohistochemistry (1-5 ug/mL)
    Immunofluorescence (20 ug/mL)
    Flow Cytometry (20 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • Lyophilized from PBS.
  • Storage Instruction:
  • Store at -20°C.
    Reconstitute in 100 uL of sterile water. Centrifuge to remove any insoluble material. After reconstitution with deionized water, store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • IHC (fresh, acetone fixed sections only, epitope is fixation sensitive), not suitable in paraffin embedded tissues. This antibody is not recommended for denaturing WB applications.
  • Publication Reference
  • Applications
  • Immunohistochemistry
  • Immunohistochemistry
  • Immunohistochemical staining of NGFR in Balb/C mouse brain (septum) using NGFR monoclonal antibody, clone MLR2 (Cat # MAB0291). 4% paraformaldehyde fixed mouse brain free floating sections were incubated with NGFR monoclonal antibody, clone MLR2 (1 ug / mL) overnight, followed by incubation with biotinylated goat anti-mouse IgG conjugate at a dilution of 1 : 500 and Vector ABC, DAB stained.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescent detection of NGFR in cultured mouse NSC-34 cells using a NGFR monoclonal antibody, clone MLR2 (Cat # MAB0291).
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence staining of adult mouse basal forebrain cholinergic neurons with NGFR monoclonal antibody, clone MLR2 (Cat # MAB0291), with ALEXA488 secondary antibodies.
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometry analysis of mouse neural progenitor cells differentiated from mES (Red curve). PE-labelled goat anti-mouse IgG was used as secondary antibody (Blue curve). Negative control processed with secondary antibody only. Data was acquired on Moxi FlowTM.
  • Application Image
  • Gene Information
  • Entrez GeneID:
  • 4804
  • Gene Name:
  • NGFR
  • Gene Alias:
  • CD271,Gp80-LNGFR,TNFRSF16,p75(NTR),p75NTR
  • Gene Description:
  • nerve growth factor receptor (TNFR superfamily, member 16)
  • Gene Summary:
  • Nerve growth factor receptor contains an extracellular domain containing four 40-amino acid repeats with 6 cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain, and a 155-amino acid cytoplasmic domain. The cysteine-rich region contains the nerve growth factor binding domain. [provided by RefSeq
  • Other Designations:
  • low affinity nerve growth factor receptor,low-affinity nerve growth factor receptor,nerve growth factor receptor
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