Product Browser

Last updated: 2017/10/15
  • Related Product Showcase

Product Compare

Product Compare Cancel Click this icon to add products to compare list. Select up to 10 products.

Quick Order (Tutorial)

Input Catalog #,
place order here!
Catalog # :
  • Where to buy
  • Choose your location

NR3C1 monoclonal antibody, clone m2F8

  • Catalog # : MAB0119
  • Visit Frequency :
  • Countries :
  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinantt NR3C1.
  • Immunogen:
  • Recombinant protein corresponding to chimeric protein with amino acids 304-428 of human NR3C1 and MS2 polymerase.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Isotype:
  • IgG2b, kappa
  • Recommend Usage:
  • The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.1% BSA, 0.01% thimerosal)
  • Storage Instruction:
  • Store at -20°C. For long term storage, store at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains thimerosal: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • ChIP
  • ChIP
  • ChIP results obtained with the antibody directed against NR3C1 monoclonal antibody, clone m2F8 (Cat # MAB0119). ChIP assays were performed using HeLa cells.
  • ChIP on chip
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Analysis by Western blot of cell extracts using the NR3C1 monoclonal antibody, clone m2F8 (Cat # MAB0119).
    A) Analysis by Western blot of extracts from HeLa cells containing the indicated amounts of Nr3C1 (from 30 to 240 fmol) and from 5x 106 Raji cells and 5x 106 Molt cells using the MAb directed against human NR3C1.
    B) 300,000 HeLa cells were also analyzed by Western blot using the MAb anti-NR3C1. The monoclonal antibody directed against NR3C1 monoclonal antibody, clone m2F8 (Cat # MAB0119) is used at 1 ug/mL .
  • Immunohistochemistry
  • Immunohistochemistry
  • Immunohistochemistry using the NR3C1 monoclonal antibody, clone m2F8 (Cat # MAB0119) at 2.5 ug/mL . Here we show NR3C1 immunoreactivity in rat CA1 neurons of hippocampus.
  • Immunocytochemistry
  • Immunocytochemistry
  • Immunocytochemistry using the NR3C1 monoclonal antibody, clone m2F8 (Cat # MAB0119). COS-7 cells transiently overexpressing human NR3C1 were labeled with the NR3C1 monoclonal antibody, clone m2F8 (Cat # MAB0119) at 2.5 ug/mL followed by biotinylated second antibody and peroxidase-labeled avidin.
  • Immunoprecipitation
  • Immunoprecipitation
  • Immunoprecipitation using the NR3C1 monoclonal antibody, clone m2F8 (Cat # MAB0119). The immunoprecipitation (IP) of the glucocorticoid receptor from HeLa cell extracts is performed using the monoclonal antibody directed against NR3C1 as follows : 5 ug of antibody per 5 million HeLa cells in 100 uL IP reaction solution. The IP is followed by Western blot analysis with the same antibody.
  • Sandwich ELISA
  • Sandwich ELISA
  • Sandwich ELISA of NR3C1 monoclonal antibody, clone m2F8 (Cat # MAB0119).
    A) Schematic representation of the sandwich ELISA, the NR3C1 monoclonal antibody, clone m2F8 (Cat # MAB0119) is used at 0.5 ug/mL .
    B) As increasing amounts of recombinant NR3C1 are used in the ELISA, the ELISA signal is proportionally increasing.
  • Gel Supershift Assay
  • Flow Cytometry
  • Application Image
  • ChIP on chip
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Gel Supershift Assay
  • Flow Cytometry
  • Gene Information
  • Entrez GeneID:
  • 2908
  • Gene Name:
  • NR3C1
  • Gene Alias:
  • GCCR,GCR,GR,GRL
  • Gene Description:
  • nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor)
  • Gene Summary:
  • The protein encoded by this gene is a receptor for glucocorticoids that can act as both a transcription factor and as a regulator of other transcription factors. This protein can also be found in heteromeric cytoplasmic complexes along with heat shock factors and immunophilins. The protein is typically found in the cytoplasm until it binds a ligand, which induces transport into the nucleus. Mutations in this gene are a cause of glucocorticoid resistance, or cortisol, resistance. Alternate splicing, the use of at least three different promoters, and alternate translation initiation sites result in several transcript variants encoding the same protein or different isoforms, but the full-length nature of some variants has not been determined. [provided by RefSeq
  • Other Designations:
  • nuclear receptor subfamily 3, group C, member 1
  • Related Disease
  • RSS
  • YouTube
  • Linkedin
  • Facebook