TLR5 monoclonal antibody, clone 19D759.2
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of TLR5 in A) Ramos and B) Raw cell lysate using TLR5 monoclonal antibody, clone 19D759.2 (Cat # MAB0109) at 2 ug/mL .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of TLR5 in formalin-fixed, paraffin-embedded mouse brain tissue using an isotype control (top) and TLR5 monoclonal antibody, clone 19D759.2 (Cat # MAB0109) (bottom) at 5 ug/mL .
Flow Cytometry
Intracellular flow analysis of TLR5 in Ramos cells. Using 1 ug of TLR5 monoclonal antibody, clone 19D759.2 (Cat # MAB0109). Shaded histogram represents cells without antibody; Green represents isotype control (BD Biosciences) ; orange represents TLR5 (Cat # MAB0109) antibody. Secondary antibody Goat Anti-Mouse IgG PE.
Flow Cytometry
Intracellular flow analysis of TLR5 in human PBMC (monocytes) using 0.5 ug of TLR5 monoclonal antibody, clone 19D759.2 (Cat # MAB0109). Shaded histogram represents cells without antibody; Green represents isotype control (BD Biosciences) ; red represents TLR5 (Cat # MAB0109). Secondary antibody Goat Anti-Mouse IgG PE.
-
Specification
Product Description
Mouse monoclonal antibody raised against synthetic peptide of TLR5.
Immunogen
A synthetic peptide corresponding to amino acids 700-800 of human TLR5.
Host
Mouse
Reactivity
Human, Mouse
Form
Liquid
Isotype
IgG2a
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In PBS (0.05% BSA, 0.05% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Cell lysate)
Western blot analysis of TLR5 in A) Ramos and B) Raw cell lysate using TLR5 monoclonal antibody, clone 19D759.2 (Cat # MAB0109) at 2 ug/mL .Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of TLR5 in formalin-fixed, paraffin-embedded mouse brain tissue using an isotype control (top) and TLR5 monoclonal antibody, clone 19D759.2 (Cat # MAB0109) (bottom) at 5 ug/mL .Flow Cytometry
Intracellular flow analysis of TLR5 in Ramos cells. Using 1 ug of TLR5 monoclonal antibody, clone 19D759.2 (Cat # MAB0109). Shaded histogram represents cells without antibody; Green represents isotype control (BD Biosciences) ; orange represents TLR5 (Cat # MAB0109) antibody. Secondary antibody Goat Anti-Mouse IgG PE.Flow Cytometry
Intracellular flow analysis of TLR5 in human PBMC (monocytes) using 0.5 ug of TLR5 monoclonal antibody, clone 19D759.2 (Cat # MAB0109). Shaded histogram represents cells without antibody; Green represents isotype control (BD Biosciences) ; red represents TLR5 (Cat # MAB0109). Secondary antibody Goat Anti-Mouse IgG PE. -
Gene Info — TLR5
Entrez GeneID
7100Gene Name
TLR5
Gene Alias
FLJ10052, MGC126430, MGC126431, SLEB1, TIL3
Gene Description
toll-like receptor 5
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene product is expressed in myelomonocytic cells, and recognizes bacterial flagellin, a principal component of bacterial flagella and a virulence factor. The activation of this receptor mobilizes the nuclear factor NF-kappaB and stimulates tumour necrosis factor-alpha production. [provided by RefSeq
Other Designations
OTTHUMP00000036039|Toll/interleukin-1 receptor-like protein 3
-
Interactome
-
Pathway
-
Disease
-
Publication Reference
-
Stimulation by TLR5 modulates osteoclast differentiation through STAT1/IFN-beta.
Ha H, Lee JH, Kim HN, Kwak HB, Kim HM, Lee SE, Rhee JH, Kim HH, Lee ZH.
Journal of Immunology 2008 Feb; 180(3):1382.
-
Intracellular signaling mechanisms regulating toll-like receptor-mediated activation of eosinophils.
Wong CK, Cheung PF, Ip WK, Lam CW.
American Journal of Respiratory Cell and Molecular Biology 2007 Mar; 37(1):85.
-
Human CD4+ T cells express TLR5 and its ligand flagellin enhances the suppressive capacity and expression of FOXP3 in CD4+CD25+ T regulatory cells.
Crellin NK, Garcia RV, Hadisfar O, Allan SE, Steiner TS, Levings MK.
Journal of Immunology 2005 Dec; 175(12):8051.
-
TGF-alpha regulates TLR expression and function on epidermal keratinocytes.
Lloyd S Miller , Ole E Sørensen, Philip T Liu, H Ray Jalian, Deborah Eshtiaghpour, Behnaz E Behmanesh, Woosin Chung, Timothy D Starner, Jenny Kim, Peter A Sieling, Tomas Ganz, Robert L Modlin.
The Journal of Immunology 2005 May; 174(10):6137.
Application：IHC-Fr, Human, Skin.
-
Inhibition of neutrophil apoptosis by TLR agonists in whole blood: involvement of the phosphoinositide 3-kinase/Akt and NF-kappaB signaling pathways, leading to increased levels of Mcl-1, A1, and phosphorylated Bad.
Francois S, El Benna J, Dang PM, Pedruzzi E, Gougerot-Pocidalo MA, Elbim C.
Journal of Immunology 2005 Mar; 174(6):3633.
-
Stimulation by TLR5 modulates osteoclast differentiation through STAT1/IFN-beta.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com