FOXP3 monoclonal antibody, clone 3G3
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis of FOXP3 in 1) full-length transfected and 2) mock transfected 293 cell lysate. Using FOXP3 monoclonal antibody, clone 3G3 (Cat # MAB0107) at 2 ug/mL.
Flow Cytometry
Flow cytometric detection of FOXP3 in approximately 2x106 mouse lymph node cells using 100 ng (in 50 uL total volume) of FOXP3 monoclonal antibody, clone 3G3 (Cat # MAB0107).
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Specification
Product Description
Mouse monoclonal antibody raised against full length recombinant Foxp3.
Immunogen
Recombinant His fusion protein corresponding to full length mouse Foxp3.
Host
Mouse
Reactivity
Human, Mouse
Form
Liquid
Isotype
IgG1, kappa
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In PBS (0.05% BSA, 0.05% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of FOXP3 in 1) full-length transfected and 2) mock transfected 293 cell lysate. Using FOXP3 monoclonal antibody, clone 3G3 (Cat # MAB0107) at 2 ug/mL.Flow Cytometry
Flow cytometric detection of FOXP3 in approximately 2x106 mouse lymph node cells using 100 ng (in 50 uL total volume) of FOXP3 monoclonal antibody, clone 3G3 (Cat # MAB0107). -
Gene Info — Foxp3
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Publication Reference
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Single-cell analysis of normal and FOXP3-mutant human T cells: FOXP3 expression without regulatory T cell development.
Gavin MA, Torgerson TR, Houston E, DeRoos P, Ho WY, Stray-Pedersen A, Ocheltree EL, Greenberg PD, Ochs HD, Rudensky AY.
PNAS 2006 Apr; 103(17):6659.
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Single-cell analysis of normal and FOXP3-mutant human T cells: FOXP3 expression without regulatory T cell development.
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