Western blot analysis of cleaved PARP1 in staurosporin-treated Jurkat cells at various time points, using PARP1 monoclonal antibody, clone 194C1439 (Cat # MAB0097) at 2 ug/mL . The band corresponding to cleaved PARP1 is only seen in the treated samples.
This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq