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Last updated: 2018/1/14
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TLR9 monoclonal antibody, clone 26C593.2

  • Catalog # : MAB0087
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against synthetic peptide of TLR9.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to amino acids 268-300 of human TLR9 isoform A.
  • Host:
  • Mouse
  • Reactivity:
  • Human, Monkey, Mouse, Rat
  • Form:
  • Liquid
  • Purification:
  • Protein G purification
  • Isotype:
  • IgG1, kappa
  • Recommend Usage:
  • ELISA (1:100-1:2000)
    Flow Cytometry
    Immunocytochemistry (1:10-1:500)
    Immunofluorescence (1:10-1:500)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (5 ug/mL)
    Immunoprecipitation (1:10-1:500)
    Western Blot (2-5 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.05% sodium azide).
  • Storage Instruction:
  • Store at 4°C for short term. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot
  • Western Blot
  • Western blot analysis of TLR9 in A) human PBMC, B) human intestine, C) mouse intestine and D) rat intestine tissue lysates using TLR9 monoclonal antibody, clone 26C593.2 (Cat # MAB0087) at 3 ug/mL .
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • IHC analysis of TLR9 monoclonal antibody, clone 26C593.2 (Cat # MAB0087) in adenocarcinoma of the lung (A) , A-549 cells (C) , and malignant lung tissues (F) at 1 : 100 dilution.
  • Immunohistochemistry
  • Immunocytochemistry
  • Immunofluorescence
  • Immunoprecipitation
  • ELISA
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of TLR9 in Ramos cells. Using 0.1 ug of TLR9 monoclonal antibody, clone 26C593.2 (Cat # MAB0087). Shaded histogram represents Ramos cells without antibody; Green represents isotype control ; red represents anti-TLR9 antibody.
  • Application Image
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Immunohistochemistry
  • Immunocytochemistry
  • Immunofluorescence
  • Immunoprecipitation
  • ELISA
  • Gene Information
  • Protein Accession#:
  • AAF78037; Q9NR96
  • Gene Name:
  • TLR9
  • Gene Alias:
  • CD289
  • Gene Description:
  • toll-like receptor 9
  • Gene Summary:
  • The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is preferentially expressed in immune cell rich tissues, such as spleen, lymph node, bone marrow and peripheral blood leukocytes. Studies in mice and human indicate that this receptor mediates cellular response to unmethylated CpG dinucleotides in bacterial DNA to mount an innate immune response. [provided by RefSeq
  • Other Designations:
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