Dnmt3a monoclonal antibody, clone 64B1446
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis of Dnmt3a in (A) Dnmt3a transfected 293 cell lysate and (B) untransfected 293 cell lysate. Using Dnmt3a monoclonal antibody, clone 64B1446 (Cat # MAB0080) at 1 ug/mL .
Immunocytochemistry
Immuno-staining of Dnmt3a. Using Dnmt3a monoclonal antibody, clone 64B1446 (Cat # MAB0080) at 5 ug/mL dilution on cultured HeLa cells. The nucleic staining is shown in most of the cells.
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Specification
Product Description
Mouse monoclonal antibody raised against full length recombinant Dnmt3a.
Immunogen
Recombinant His fusion protein corresponding to full length mouse Dnmt3a.
Host
Mouse
Reactivity
Human, Mouse
Form
Liquid
Isotype
IgG1
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In PBS (0.05% BSA, 0.05% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
ChIP
Western Blot (Transfected lysate)
Western blot analysis of Dnmt3a in (A) Dnmt3a transfected 293 cell lysate and (B) untransfected 293 cell lysate. Using Dnmt3a monoclonal antibody, clone 64B1446 (Cat # MAB0080) at 1 ug/mL .Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunocytochemistry
Immuno-staining of Dnmt3a. Using Dnmt3a monoclonal antibody, clone 64B1446 (Cat # MAB0080) at 5 ug/mL dilution on cultured HeLa cells. The nucleic staining is shown in most of the cells.Immunofluorescence
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Gene Info — Dnmt3a
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Publication Reference
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SALL3 expression balance underlies lineage biases in human induced pluripotent stem cell differentiation.
Kuroda T, Yasuda S, Tachi S, Matsuyama S, Kusakawa S, Tano K, Miura T, Matsuyama A, Sato Y.
Nature Communications 2019 May; 10(1):2175.
Application：WB-Ce, Human, 253G1 cells.
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Methylation of LOXL1 promoter by DNMT3A in aged human skin fibroblasts.
Moulin L, Cenizo V, Nyamay Antu A, Andre-Frei V, Pain S, Sommer P, Debret R.
Rejuvenation Research 2017 Apr; 20(2):103.
Application：IP, Human, Dermal fibroblasts.
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Arsenic induces functional re-expression of estrogen receptor α by demethylation of DNA in estrogen receptor-negative human breast cancer.
Du J, Zhou N, Liu H, Jiang F, Wang Y, Hu C, Qi H, Zhong C, Wang X, Li Z.
PLoS One 2012 Apr; 7(4):e35957.
Application：WB-Ce, Human, MCF-7, MDA-MB-231 cells.
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Multiplex rt-PCR expression analysis of developmentally important genes in individual mouse preimplantation embryos and blastomeres.
May A, Kirchner R, Muller H, Hartmann P, El Hajj N, Tresch A, Zechner U, Mann W, Haaf T.
Biology of Reproduction 2009 Jan; 80(1):194.
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Maternal and zygotic Dnmt1 are necessary and sufficient for the maintenance of DNA methylation imprints during preimplantation development.
Hirasawa R, Chiba H, Kaneda M, Tajima S, Li E, Jaenisch R, Sasaki H.
Genes & Development 2008 Jun; 22(12):1607.
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Helicobacter pylori regulates p21(WAF1) by histone H4 acetylation.
Xia G, Schneider-Stock R, Diestel A, Habold C, Krueger S, Roessner A, Naumann M, Lendeckel U.
Biochemical and Biophysical Research Communications 2008 Feb; 369(2):526.
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Dnmt3b promotes tumorigenesis in vivo by gene-specific de novo methylation and transcriptional silencing.
Linhart HG, Lin H, Yamada Y, Moran E, Steine EJ, Gokhale S, Lo G, Cantu E, Ehrich M, He T, Meissner A, Jaenisch R.
Genes & Development 2007 Dec; 21(23):3110.
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Coordinate regulation of DNA methyltransferase expression during oogenesis.
Lucifero D, La Salle S, Bourc'his D, Martel J, Bestor TH, Trasler JM.
BMC Developmental Biology 2007 Apr; 7:36.
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Aberrant epigenetic modifications in hepatocarcinogenesis induced by hepatitis B virus X protein.
Park IY, Sohn BH, Yu E, Suh DJ, Chung YH, Lee JH, Surzycki SJ, Lee YI.
Gastroenterology 2007 Apr; 132(4):1476.
Application：WB-Tr, Human, CHL cells.
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Dnmt3a2 targets endogenous Dnmt3L to ES cell chromatin and induces regional DNA methylation.
Nimura K, Ishida C, Koriyama H, Hata K, Yamanaka S, Li E, Ura K, Kaneda Y.
Genes to Cells 2006 Oct; 11(10):1225.
Application：WB, Human, Wild-type, Dnmt3a/Dnmt3b double mutant Dnmt3L-null ES cells.
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Maintenance of self-renewal ability of mouse embryonic stem cells in the absence of DNA methyltransferases Dnmt1, Dnmt3a and Dnmt3b.
Akiko Tsumura, Tomohiro Hayakawa, Yuichi Kumaki, Shin-ichiro Takebayashi, Morito Sakaue, Chisa Matsuoka, Kunitada Shimotohno, Fuyuki Ishikawa, En Li, Hiroki R Ueda, Jun-ichi Nakayama, Masaki Okano.
Genes to Cells 2006 Jul; 11(7):805.
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Dimethyl sulfoxide has an impact on epigenetic profile in mouse embryoid body.
Misa Iwatani, Kohta Ikegami, Yuliya Kremenska, Naka Hattori, Satoshi Tanaka, Shintaro Yagi, Kunio Shiota.
Stem Cells 2006 Nov; 24(11):2549.
Application：WB-Ce, Mouse, Mouse embryonic stem cells.
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Stage-specific induction of DNA methyltransferases in olfactory receptor neuron development.
Jessica L MacDonald, Christopher S Y Gin, A Jane Roskams.
Developmental Biology 2005 Dec; 288(2):461.
Application：IF, IHC, Mouse, Mouse olfactory epithelium, OP27 cells.
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Inhibition of DNA methyltransferase reverses cisplatin induced drug resistance in murine neuroblastoma cells.
Yi-Yong Qiu, Bernard L Mirkin, Rama S Dwivedi.
Cancer Detection and Prevention 2005 Sep; 29(5):456.
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De novo DNA methyltransferases Dnmt3a and Dnmt3b primarily mediate the cytotoxic effect of 5-aza-2'-deoxycytidine.
Masahiro Oka, Amy M Meacham, Takashi Hamazaki, Nemanja Rodic, Lung-Ji Chang, Naohiro Terada.
Oncogene 2004 Apr; 24(19):3091.
Application：WB-Tr, Monkey, Mouse, COS-7 cells, Mouse embryonic stem cell lines J1.
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Dynamic expression of de novo DNA methyltransferases Dnmt3a and Dnmt3b in the central nervous system.
Jian Feng, Hua Chang, En Li, Guoping Fan.
Journal of Neuroscience Research 2005 Mar; 79(6):734.
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Cell and stage of transformation-specific effects of folate deficiency on methionine cycle intermediates and DNA methylation in an in vitro model.
Joanne M Stempak, Kyoung-Jin Sohn, En-Pei Chiang, Barry Shane, Young-In Kim.
Carcinogenesis 2005 May; 26(5):981.
Application：WB-Ce, Human, Mouse, Caco-2, HCT-116, NIH/3T3 cells.
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Myc represses transcription through recruitment of DNA methyltransferase corepressor.
Carmen Brenner, Rachel Deplus, Celine Didelot, Axelle Loriot, Emmanuelle Vire, Charles De Smet, Arantxa Gutierrez, Davide Danovi, David Bernard, Thierry Boon, Pier Giuseppe Pelicci, Bruno Amati, Tony Kouzarides, Yvan de Launoit, Luciano Di Croce, Francois Fuks.
The EMBO Journal 2005 Jan; 24(2):336.
Application：ChIP, IP, WB-Ce, WB-Tr, Human, HEK 293, HeLa, U-2 OS cells.
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Loss of estrogen receptor signaling triggers epigenetic silencing of downstream targets in breast cancer.
Yu-Wei Leu, Pearlly S Yan, Meiyun Fan, Victor X Jin, Joseph C Liu, Edward M Curran, Wade V Welshons, Susan H Wei, Ramana V Davuluri, Christoph Plass, Kenneth P Nephew, Tim H-M Huang.
Cancer Research 2004 Nov; 64(22):8184.
Application：ChIP, Human, MCF-7 cells.
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Role of the DNA methyltransferase variant DNMT3b3 in DNA methylation.
Daniel J Weisenberger, Mihaela Velicescu, Jonathan C Cheng, Felicidad A Gonzales, Gangning Liang, Peter A Jones.
Molecular Cancer Research 2004 Jan; 2(1):62.
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Association of Lsh, a regulator of DNA methylation, with pericentromeric heterochromatin is dependent on intact heterochromatin.
Qingsheng Yan, Edward Cho, Stephen Lockett, Kathrin Muegge.
Molecular and Cellular Biology 2003 Dec; 23(23):8416.
Application：WB-Ce, Mouse, NIH/3T3 cells.
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Establishment and maintenance of genomic methylation patterns in mouse embryonic stem cells by Dnmt3a and Dnmt3b.
Taiping Chen, Yoshihide Ueda, Jonathan E Dodge, Zhenjuan Wang, En Li.
Molecular and Cellular Biology 2003 Aug; 23(16):5594.
Application：WB-Tr, Mouse, J1 cells.
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A novel Dnmt3a isoform produced from an alternative promoter localizes to euchromatin and its expression correlates with active de novo methylation.
Taiping Chen, Yoshihide Ueda, Shaoping Xie, En Li.
The Journal of Biological Chemistry 2002 Oct; 277(41):38746.
Application：IP, WB-Ce, WB-Tr, Bacteria, Human, Monkey, Mouse, Bacterial cells, BT-549, COS-7, ES2, Hs578T, MCF-7, MDA-MB-231, MDA-MB-435, MDAH2774, NCCIT, NIH/3T3, OV1063, OVCA-3 cells.
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Lsh, a member of the SNF2 family, is required for genome-wide methylation.
K Dennis, T Fan, T Geiman, Q Yan, K Muegge.
Genes & Development 2001 Nov; 15(22):2940.
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SALL3 expression balance underlies lineage biases in human induced pluripotent stem cell differentiation.
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