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Last updated: 2017/6/25
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DNMT1 monoclonal antibody, clone 60B1220.1

  • Catalog # : MAB0079
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against synthetic peptide of DNMT1.
  • Immunogen:
  • A synthetic peptide corresponding to amino acids 637-650 of human DNMT1.
  • Sequence:
  • EKDDREDKENAFKR
  • Host:
  • Mouse
  • Reactivity:
  • Human, Mouse
  • Form:
  • Liquid
  • Purification:
  • Protein G purification
  • Isotype:
  • IgG1, kappa
  • Recommend Usage:
  • ChIP (1:20-1:1000)
    Flow Cytometry
    Immunocytochemistry (1:10)
    Immunofluorescence (1:10)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-1:500)
    Immunohistochemistry (Free floating sections) (1:500)
    Immunohistochemistry (Frozen sections) (1:500)
    Immunoprecipitation (1:10-1:500)
    Western Blot (0.1-0.5 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.05% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • ChIP
  • Immunohistochemistry (Free floating sections)
  • Western Blot
  • Western Blot
  • Western blot analysis with DNMT1 monoclonal antibody, clone 60B1220.1 (Cat # MAB0079).
    Lane 1: 2102EP (human embryonic carcinoma) in the absence of immunizing peptide
    Lane 2: 2102EP (human embryonic carcinoma) in the presence of immunizing peptide
    Lane 3: Recombinant human Dnmt1 protein in the absence of immunizing peptide
    Lane 4: Recombinant human Dnmt1 protein in the presence of immunizing peptide
    Lane 5: NIH 3T3 (mouse embryonic fribroblast) in the absence of immunizing peptide
    Lane 6: NIH 3T3 (mouse embryonic fribroblast) in the presence of immunizing peptide
    Lane 7: D3 (mouse embryonic stem cell) in the absence of immunizing peptide
    Lane 8: D3 (mouse embryonic stem cell) in the presence of immunizing peptide
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human hepatocellular carcinoma with DNMT1 monoclonal antibody, clone 60B1220.1 (Cat # MAB0079).
  • Immunohistochemistry (Frozen sections)
  • Immunocytochemistry
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescent staining of HeLa cells with DNMT1 monoclonal antibody, clone 60B1220.1 (Cat # MAB0079) with Dylight 488 (Green). Nuclei and alpha-tubulin were counterstained with DAPI (Blue) and Dylight 550 (Red). An antibody dilution of 1:10 was used.
  • Immunoprecipitation
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of HeLa cells with DNMT1 monoclonal antibody, clone 60B1220.1 (Cat # MAB0079) (Blue). Orange: isotype control.
  • Application Image
  • ChIP
  • Immunohistochemistry (Free floating sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Immunohistochemistry (Frozen sections)
  • Immunocytochemistry
  • Immunoprecipitation
  • Gene Information
  • Entrez GeneID:
  • 1786
  • Gene Name:
  • DNMT1
  • Gene Alias:
  • AIM,CXXC9,DNMT,FLJ16293,MCMT,MGC104992
  • Gene Description:
  • DNA (cytosine-5-)-methyltransferase 1
  • Gene Summary:
  • DNA (cytosine-5-)-methyltransferase 1 has a role in the establishment and regulation of tissue-specific patterns of methylated cytosine residues. Aberrant methylation patterns are associated with certain human tumors and developmental abnormalities. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
  • Other Designations:
  • CXXC finger protein 9,DNA methyltransferase 1
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