TERF2 monoclonal antibody, clone 4A794.15
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More Files
- More Functions
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Specification
Product Description
Mouse monoclonal antibody raised against full length recombinant TERF2.
Immunogen
Recombinant protein corresponding to full length human TERF2.
Host
Mouse
Reactivity
Deer, Human, Mouse, Rat
Form
Liquid
Isotype
IgG1
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In PBS (0.05% BSA, 0.05% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Frozen sections)
Immunocytochemistry
Immunofluorescence
Immunoprecipitation
Immunoprecipitation (IP) / Western Blot (WB) Analysis of TERF2 in HL-60 cells : (Lane 1) IP with mouse TERF2 monoclonal antibody, clone 4A794.15 (Cat # MAB0074) . (Lane 2) IP with control mouse IgG. (Lane 3) IP with goat anti-TRF2. (Lane 4) IP with pre-immune goat Ig. (Lanes 1-4) WB with TERF2 monoclonal antibody, clone 4A794.15 (Cat # MAB0074) . TRF2 is detected as an ~ 66 kD protein.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
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Gene Info — TERF2
Entrez GeneID
7014Gene Name
TERF2
Gene Alias
TRBF2, TRF2
Gene Description
telomeric repeat binding factor 2
Omim ID
602027Gene Ontology
HyperlinkGene Summary
This gene encodes a telomere specific protein, TERF2, which is a component of the telomere nucleoprotein complex. This protein is present at telomeres in metaphase of the cell cycle, is a second negative regulator of telomere length and plays a key role in the protective activity of telomeres. While having similar telomere binding activity and domain organization, TERF2 differs from TERF1 in that its N terminus is basic rather than acidic. [provided by RefSeq
Other Designations
telomeric repeat binding protein 2
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Interactome
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Disease
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Publication Reference
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Telomere elongation by a mutant tankyrase 1 without TRF1 poly(ADP-ribosyl)ation.
Muramatsu Y, Tahara H, Ono T, Tsuruo T, Seimiya H.
Experimental Cell Research 2008 Mar; 314(5):1115.
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The telomeric protein TRF2 is critical for the protection of A549 cells from both telomere erosion and DNA double-strand breaks driven by salvicine.
Zhang YW, Zhang ZX, Miao ZH, Ding J.
Molecular Pharmacology 2007 Nov; 73(3):824.
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FLASH is required for histone transcription and S-phase progression.
Barcaroli D, Bongiorno-Borbone L, Terrinoni A, Hofmann TG, Rossi M, Knight RA, Matera AG, Melino G, De Laurenzi V.
PNAS 2006 Sep; 103(40):14808.
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Changes in lamina structure are followed by spatial reorganization of heterochromatic regions in caspase-8-activated human mesenchymal stem cells.
Raz V, Carlotti F, Vermolen BJ, van der Poel E, Sloos WC, Knaan-Shanzer S, de Vries AA, Hoeben RC, Young IT, Tanke HJ, Garini Y, Dirks RW.
Journal of Cell Science 2006 Sep; 119(Pt 20):4247.
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TRF2 inhibition triggers apoptosis and reduces tumourigenicity of human melanoma cells.
Biroccio A, Rizzo A, Elli R, Koering CE, Belleville A, Benassi B, Leonetti C, Stevens MF, D'Incalci M, Zupi G, Gilson E.
European Journal of Cancer 2006 Jun; 42(12):1881.
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PML nuclear bodies are highly organised DNA-protein structures with a function in heterochromatin remodelling at the G2 phase.
Judith J Luciani, Danielle Depetris, Yves Usson, Catherine Metzler-Guillemain, Cecile Mignon-Ravix, Micheal J Mitchell, Andre Megarbane, Pierre Sarda, Huseyin Sirma, Anne Moncla, Jean Feunteun, Marie-Genevieve Mattei.
Journal of Cell Science 2006 Jun; 119(Pt 12):2518.
Application:IF, Human, ICF, WI-38 cells.
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hSnm1B is a novel telomere-associated protein.
Brian D Freibaum, Christopher M Counter.
The Journal of Biological Chemistry 2006 Jun; 281(22):15033.
Application:IF, WB-Tr, Human, HEK 293T cells.
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DNA damage-induced phosphorylation of the human telomere-associated protein TRF2.
Hiromi Tanaka, Marc S Mendonca, Paul S Bradshaw, Derek J Hoelz, Linda H Malkas, M Stephen Meyn, David Gilley.
PNAS 2005 Oct; 102(43):15539.
Application:IF, IP, WB-Ce, Human, HeLa, HT-1080 cells, Human primary normal fibroblast BJ cells.
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Multiple mechanisms of telomere maintenance exist in liposarcomas.
Jay E Johnson, Robert J Varkonyi, Jaclyn Schwalm, Ryan Cragle, Andres Klein-Szanto, Arthur Patchefsky, Edna Cukierman, Margaret von Mehren, Dominique Broccoli.
Clinical Cancer Research 2005 Aug; 11(15):5347.
Application:IF, IHC-Fr, Human, HeLa, HIO107 cells, Human liposarcomas.
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Characterization of the telomere complex, TERF1 and TERF2 genes in muntjac species with fusion karyotypes.
Nils Hartmann, Harry Scherthan.
Experimental Cell Research 2005 May; 306(1):64.
Application:IF, Deer, Deer chromosomes.
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Accumulation and altered localization of telomere-associated protein TRF2 in immortally transformed and tumor-derived human breast cells.
Tarlochan Nijjar, Ekaterina Bassett, James Garbe, Yasuhiro Takenaka, Martha R Stampfer, David Gilley, Paul Yaswen.
Oncogene 2005 May; 24(20):3369.
Application:IF, IHC-P, WB-Ce, Human, Human breast tissues, Human mammary epithelial cells, MDA-MB-468, T-47D cells.
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Higher-order nuclear organization in growth arrest of human mammary epithelial cells: a novel role for telomere-associated protein TIN2.
Patrick Kaminker, Cedric Plachot, Sahn-Ho Kim, Peter Chung, Danielle Crippen, Ole W Petersen, Mina J Bissell, Judith Campisi, Sophie A Lelievre.
Journal of Cell Science 2005 Mar; 118(Pt 6):1321.
Application:IF, Human, Human mammary epithelial cells.
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Human telomeric protein TRF2 associates with genomic double-strand breaks as an early response to DNA damage.
Paul S Bradshaw, Dimitrios J Stavropoulos, M Stephen Meyn.
Nature Genetics 2005 Feb; 37(2):193.
Application:IF, WB-Tr, Human, Human fibroblasts, IMR90 cells.
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Tankyrase 1 as a target for telomere-directed molecular cancer therapeutics.
Seimiya H, Muramatsu Y, Ohishi T, Tsuruo T.
Cancer Cell 2005 Jan; 7(1):25.
Application:WB, Human, HT-1080, HTC75 cells.
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Regulation of WRN helicase activity in human base excision repair.
Byungchan Ahn, Jeanine A Harrigan, Fred E Indig, David M Wilson 3rd, Vilhelm A Bohr.
The Journal of Biological Chemistry 2004 Dec; 279(51):53465.
Application:IF, Human, U-2 osteosarcoma cells.
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A dynamic molecular link between the telomere length regulator TRF1 and the chromosome end protector TRF2.
Houghtaling BR, Cuttonaro L, Chang W, Smith S.
Current Biology 2004 Sep; 14(18):1621.
Application:IP, WB-Tr, Human, HTC75 cells.
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TIN2 mediates functions of TRF2 at human telomeres.
Sahn-ho Kim, Christian Beausejour, Albert R Davalos, Patrick Kaminker, Seok-Jin Heo, Judith Campisi.
The Journal of Biological Chemistry 2004 Oct; 279(42):43799.
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DNA polymerase beta interacts with TRF2 and induces telomere dysfunction in a murine mammary cell line.
Poppy Fotiadou, Octavian Henegariu, Joann B Sweasy.
Cancer Research 2004 Jun; 64(11):3830.
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Functional subdomain in the ankyrin domain of tankyrase 1 required for poly(ADP-ribosyl)ation of TRF1 and telomere elongation.
Hiroyuki Seimiya, Yukiko Muramatsu, Susan Smith, Takashi Tsuruo.
Molecular and Cellular Biology 2004 Mar; 24(5):1944.
Application:IF, WB-Tr, Human, HeLa, HTC75 cells.
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Telomere repeat binding factors TRF1, TRF2, and hRAP1 modulate replication of Epstein-Barr virus OriP.
Zhong Deng, Constandache Atanasiu, John S Burg, Dominique Broccoli, Paul M Lieberman.
Journal of Virology 2003 Nov; 77(22):11992.
Application:ChIP, WB-Ce, WB-Tr, Human, Raji, ZKO-293 cells.
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The Bloom syndrome helicase BLM interacts with TRF2 in ALT cells and promotes telomeric DNA synthesis.
Dimitrios J Stavropoulos, Paul S Bradshaw, Xiaobin Li, Ivan Pasic, Kevin Truong, Mitsuhiko Ikura, Mark Ungrin, M Stephen Meyn.
Human Molecular Genetics 2002 Dec; 11(25):3135.
Application:IF, WB-Tr, Human, GM00847, GM01161, WI38 cells.
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Telomere-binding protein TRF2 binds to and stimulates the Werner and Bloom syndrome helicases.
Opresko PL, von Kobbe C, Laine JP, Harrigan J, Hickson ID, Bohr VA.
The Journal of Biological Chemistry 2002 Oct; 277(43):41110.
Application:ELISA, IF, IP-WB, Human, HeLa, U2OS cells.
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Telomeric proteins regulate episomal maintenance of Epstein-Barr virus origin of plasmid replication.
Zhong Deng, Larissa Lezina, Chi-Ju Chen, Svetlana Shtivelband, Wingkan So, Paul M Lieberman.
Molecular Cell 2002 Mar; 9(3):493.
Application:ChIP, WB-Ce, WB-Tr, Human, HEK 293, HeLa, Raji, SL1 cells.
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Role for the related poly(ADP-Ribose) polymerases tankyrase 1 and 2 at human telomeres.
Brandoch D Cook, Jasmin N Dynek, William Chang, Grigoriy Shostak, Susan Smith.
Molecular and Cellular Biology 2002 Jan; 22(1):332.
Application:WB-Tr, Human, HTC75, WI-38 cells.
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The Saccharomyces cerevisiae WRN homolog Sgs1p participates in telomere maintenance in cells lacking telomerase.
Johnson FB, Marciniak RA, McVey M, Stewart SA, Hahn WC, Guarente L.
The EMBO Journal 2001 Feb; 20(4):905.
Application:IF, Human, WI38 75.1 fibroblasts.
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Mammalian meiotic telomeres: protein composition and redistribution in relation to nuclear pores.
H Scherthan, M Jerratsch, B Li, S Smith, M Hultén, T Lock, T de Lange.
Molecular Biology of the Cell 2000 Dec; 11(12):4189.
Application:IF, IHC-Fr, Human, Mouse, Human testes, Mouse Mouse spermatocytes.
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Telomere elongation by a mutant tankyrase 1 without TRF1 poly(ADP-ribosyl)ation.
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